LiveCount Assay: concomitant measurement of cytolytic activity and phenotypic characterisation of CD8(+) T-cells by flow cytometry.

Details

Serval ID
serval:BIB_C9B89A2E2AB7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
LiveCount Assay: concomitant measurement of cytolytic activity and phenotypic characterisation of CD8(+) T-cells by flow cytometry.
Journal
Journal of immunological methods
Author(s)
Devêvre E., Romero P., Mahnke Y.D.
ISSN
0022-1759
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
311
Number
1-2
Pages
31-46
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Tumour immunologists strive to develop efficient tumour vaccination and adoptive transfer therapies that enlarge the pool of tumour-specific and -reactive effector T-cells in vivo. To assess the efficiency of the various strategies, ex vivo assays are needed for the longitudinal monitoring of the patient's specific immune responses providing both quantitative and qualitative data. In particular, since tumour cell cytolysis is the end goal of tumour immunotherapy, routine immune monitoring protocols need to include a read-out for the cytolytic efficiency of Ag-specific cells. We propose to combine current immune monitoring techniques in a highly sensitive and reproducible multi-parametric flow cytometry based cytotoxicity assay that has been optimised to require low numbers of Ag-specific T-cells. The possibility of re-analysing those T-cells that have undergone lytic activity is illustrated by the concomitant detection of CD107a upregulation on the surface of degranulated T-cells. To date, the LiveCount Assay provides the only possibility of assessing the ex vivo cytolytic activity of low-frequency Ag-specific cytotoxic T-lymphocytes from patient material.
Keywords
Antigens, Neoplasm/immunology, CD8-Positive T-Lymphocytes/cytology, CD8-Positive T-Lymphocytes/immunology, Cancer Vaccines/immunology, Cell Count, Cytokines/immunology, Cytotoxicity Tests, Immunologic/methods, Flow Cytometry/methods, Histocompatibility Antigens/immunology, Humans, Immunophenotyping/methods, Immunotherapy, Adoptive/methods, Lymphocyte Activation, Neoplasms/immunology, Neoplasms/therapy, T-Lymphocytes, Cytotoxic/cytology, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Create date
28/01/2008 11:28
Last modification date
20/08/2019 15:44
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