Biochemical quantification of total brain glycogen concentration in rats under different glycemic states.

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Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_C81559D67CD6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Biochemical quantification of total brain glycogen concentration in rats under different glycemic states.
Périodique
Neurochemistry International
Auteur(s)
Morgenthaler F.D., Koski D.M., Kraftsik R., Henry P.G., Gruetter R.
ISSN
0197-0186 (Print)
ISSN-L
0197-0186
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
48
Numéro
6-7
Pages
616-622
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
All (13)C NMR studies of brain glycogen to date relied on observing the incorporation of (13)C label into glycogen, and thus interpretation was potentially affected by changes in (13)C label turnover rates. The goal of this study was to quantify total brain glycogen concentration under conditions of hypoglycemia or normoglycemia using biochemical methods. Rats were sacrificed using a focused microwave fixation device. The results showed that metabolism of brain glycogen was Glc- and insulin-sensitive and that insulin-induced hypoglycemia promoted a gradual glycogenolysis. Moreover, we show that there are very mild effects of isoflurane and alpha-chloralose anesthesia on brain glycogen concentration. Altogether these results show that total brain glycogen serves as a substantial source of glucosyl units during insulin-induced moderate hypoglycemia and therefore may be neuroprotective. Finally we also conclude that previous interpretation of (13)C NMR spectroscopy data accurately reflected the changes in total brain glycogen content.
Mots-clé
Animals, Blood Glucose/metabolism, Brain/metabolism, Glycogen/metabolism, Hypoglycemia/metabolism, Insulin/metabolism, Magnetic Resonance Spectroscopy, Male, Rats, Rats, Sprague-Dawley
Pubmed
Web of science
Création de la notice
26/02/2008 17:25
Dernière modification de la notice
03/03/2018 21:20
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