Regulation of PSGL-1 interactions with L-selectin, P-selectin, and E-selectin: role of human fucosyltransferase-IV and -VII.

Détails

ID Serval
serval:BIB_C7F00885DFDE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Regulation of PSGL-1 interactions with L-selectin, P-selectin, and E-selectin: role of human fucosyltransferase-IV and -VII.
Périodique
Journal of Biological Chemistry
Auteur(s)
Martinez M., Joffraud M., Giraud S., Baïsse B., Bernimoulin M.P., Schapira M., Spertini O.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
280
Numéro
7
Pages
5378-5390
Langue
anglais
Résumé
P-selectin glycoprotein ligand-1 (PSGL-1) interactions with selectins regulate leukocyte migration in inflammatory lesions. In mice, selectin ligand activity regulating leukocyte recruitment and lymphocyte homing into lymph nodes results from the sum of unequal contributions of fucosyltransferase (FucT)-IV and FucT-VII, with FucT-VII playing a predominant role. Here we have examined the role of human FucT-IV and -VII in conferring L-selectin, P-selectin, and E-selectin binding activities to PSGL-1. Lewis x (Le(x)) carbohydrate was generated at the CHO(dhfr)(-) cell surface by FucT-IV expression, whereas sialyl Le(x) (sLe(x)) was synthesized by FucT-VII. Both human FucT-IV and -VII had the ability to generate carbohydrate ligands that support L-selectin-, P-selectin-, and E-selectin-dependent rolling on PSGL-1, with FucT-VII playing a major role. Cooperation was observed between FucT-IV and -VII in recruiting L-, P-, or E-selectin-expressing cells on PSGL-1 and in regulating cell rolling velocity and stability. Additional rolling adhesion assays were performed to assess the role of Thr-57-linked core-2 O-glycans in supporting L-selectin-, P-selectin-, and E-selectin-dependent rolling on PSGL-1. These studies confirmed that core-2 O-glycans attached to Thr-57 play a critical role in supporting L- and P-selectin-dependent rolling and revealed that additional binding sites support >75% of E-selectin-mediated rolling. The observations presented here indicate that human FucT-IV and -VII both contribute and cooperate in regulating L-selectin-, P-selectin-, and E-selectin-dependent rolling on PSGL-1, with FucT-VII playing a predominant role in conferring selectin binding activity to PSGL-1.
Mots-clé
Animals, Antigens, CD15/chemistry, Antigens, CD15/metabolism, CHO Cells, Cell Adhesion, Cells, Cultured, Cricetinae, Epitopes/chemistry, Epitopes/metabolism, Flow Cytometry, Fucosyltransferases/genetics, Fucosyltransferases/metabolism, Glycosylation, Humans, Leukocyte Rolling, Membrane Glycoproteins/chemistry, Membrane Glycoproteins/genetics, Mice, Neutrophils/cytology, Neutrophils/metabolism, Oligosaccharides/chemistry, Oligosaccharides/metabolism, Protein Binding/drug effects, Selectins/genetics, Selectins/metabolism, Transfection
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 16:28
Dernière modification de la notice
09/05/2019 1:07
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