Regulation of PSGL-1 interactions with L-selectin, P-selectin, and E-selectin: role of human fucosyltransferase-IV and -VII.

Details

Serval ID
serval:BIB_C7F00885DFDE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Regulation of PSGL-1 interactions with L-selectin, P-selectin, and E-selectin: role of human fucosyltransferase-IV and -VII.
Journal
Journal of Biological Chemistry
Author(s)
Martinez M., Joffraud M., Giraud S., Baïsse B., Bernimoulin M.P., Schapira M., Spertini O.
ISSN
0021-9258
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
280
Number
7
Pages
5378-5390
Language
english
Abstract
P-selectin glycoprotein ligand-1 (PSGL-1) interactions with selectins regulate leukocyte migration in inflammatory lesions. In mice, selectin ligand activity regulating leukocyte recruitment and lymphocyte homing into lymph nodes results from the sum of unequal contributions of fucosyltransferase (FucT)-IV and FucT-VII, with FucT-VII playing a predominant role. Here we have examined the role of human FucT-IV and -VII in conferring L-selectin, P-selectin, and E-selectin binding activities to PSGL-1. Lewis x (Le(x)) carbohydrate was generated at the CHO(dhfr)(-) cell surface by FucT-IV expression, whereas sialyl Le(x) (sLe(x)) was synthesized by FucT-VII. Both human FucT-IV and -VII had the ability to generate carbohydrate ligands that support L-selectin-, P-selectin-, and E-selectin-dependent rolling on PSGL-1, with FucT-VII playing a major role. Cooperation was observed between FucT-IV and -VII in recruiting L-, P-, or E-selectin-expressing cells on PSGL-1 and in regulating cell rolling velocity and stability. Additional rolling adhesion assays were performed to assess the role of Thr-57-linked core-2 O-glycans in supporting L-selectin-, P-selectin-, and E-selectin-dependent rolling on PSGL-1. These studies confirmed that core-2 O-glycans attached to Thr-57 play a critical role in supporting L- and P-selectin-dependent rolling and revealed that additional binding sites support >75% of E-selectin-mediated rolling. The observations presented here indicate that human FucT-IV and -VII both contribute and cooperate in regulating L-selectin-, P-selectin-, and E-selectin-dependent rolling on PSGL-1, with FucT-VII playing a predominant role in conferring selectin binding activity to PSGL-1.
Keywords
Animals, Antigens, CD15/chemistry, Antigens, CD15/metabolism, CHO Cells, Cell Adhesion, Cells, Cultured, Cricetinae, Epitopes/chemistry, Epitopes/metabolism, Flow Cytometry, Fucosyltransferases/genetics, Fucosyltransferases/metabolism, Glycosylation, Humans, Leukocyte Rolling, Membrane Glycoproteins/chemistry, Membrane Glycoproteins/genetics, Mice, Neutrophils/cytology, Neutrophils/metabolism, Oligosaccharides/chemistry, Oligosaccharides/metabolism, Protein Binding/drug effects, Selectins/genetics, Selectins/metabolism, Transfection
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 15:28
Last modification date
20/08/2019 15:43
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