Characterization of cell cycle events during the onset of sporulation in Bacillus subtilis

Détails

ID Serval
serval:BIB_C7B72214908E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Characterization of cell cycle events during the onset of sporulation in Bacillus subtilis
Périodique
Journal of Bacteriology
Auteur(s)
Hauser  P. M., Errington  J.
ISSN
0021-9193 (Print)
Statut éditorial
Publié
Date de publication
07/1995
Volume
177
Numéro
14
Pages
3923-31
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Résumé
To elucidate the process of asymmetric division during sporulation of Bacillus subtilis, we have measured changes in cell cycle parameters during the transition from vegetative growth to sporulation. Because the propensity of B. subtilis to grow in chains of cells precludes the use of automated cell-scanning devices, we have developed a fluorescence microscopic method for analyzing cell cycle parameters in individual cells. From the results obtained, and measurements of DNA replication fork elongation rates and the escape time of sporulation from the inhibition of DNA replication, we have derived a detailed time scale for the early morphological events of sporulation which is mainly consistent with the cell cycle changes expected following nutritional downshift. The previously postulated sensitive stage in the DNA replication cycle, beyond which the cell is unable to sporulate without a new cell cycle, could represent a point in the division cycle at which the starved cell cannot avoid attaining the initiation mass for DNA replication and thus embarking on another round of the cell cycle. The final cell cycle event, formation of the asymmetric spore septum, occurs at about the time in the cell cycle at which the uninduced cell would have divided centrally, in keeping with the view that spore septation is a modified version of vegetative division.
Mots-clé
Bacillus subtilis/cytology/drug effects/*physiology Caseins/pharmacology Cell Cycle/*physiology Culture Media DNA, Bacterial/isolation & purification Histocytochemistry Image Processing, Computer-Assisted Microscopy, Fluorescence Models, Biological Morphogenesis Protein Hydrolysates/pharmacology Spores, Bacterial/cytology/physiology
Pubmed
Web of science
Création de la notice
25/01/2008 15:30
Dernière modification de la notice
03/03/2018 21:20
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