Selective in vivo visualization of immune-cell infiltration in a mouse model of autoimmune myocarditis by fluorine-19 cardiac magnetic resonance.

Détails

Ressource 1Télécharger: vanHeeswijk_F19Myocarditis_CircCVImaging2013_preprintcover.pdf (1427.78 [Ko])
Etat: Public
Version: Author's accepted manuscript
Licence: Non spécifiée
ID Serval
serval:BIB_C7B2751B340B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Selective in vivo visualization of immune-cell infiltration in a mouse model of autoimmune myocarditis by fluorine-19 cardiac magnetic resonance.
Périodique
Circulation. Cardiovascular Imaging
Auteur(s)
van Heeswijk R.B., De Blois J., Kania G., Gonzales C., Blyszczuk P., Stuber M., Eriksson U., Schwitter J.
ISSN
1942-0080 (Electronic)
ISSN-L
1941-9651
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
6
Numéro
2
Pages
277-284
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish; PDF : Original Article
Résumé
BACKGROUND: The goal of this study was to characterize the performance of fluorine-19 ((19)F) cardiac magnetic resonance (CMR) for the specific detection of inflammatory cells in a mouse model of myocarditis. Intravenously administered perfluorocarbons are taken up by infiltrating inflammatory cells and can be detected by (19)F-CMR. (19)F-labeled cells should, therefore, generate an exclusive signal at the inflamed regions within the myocardium.
METHODS AND RESULTS: Experimental autoimmune myocarditis was induced in BALB/c mice. After intravenous injection of 2×200 µL of a perfluorocarbon on day 19 and 20 (n=9) after immunization, in vivo (19)F-CMR was performed at the peak of myocardial inflammation (day 21). In 5 additional animals, perfluorocarbon combined with FITC (fluorescein isothiocyanate) was administered for postmortem immunofluorescence and flow-cytometry analyses. Control experiments were performed in 9 animals. In vivo (19)F-CMR detected myocardial inflammation in all experimental autoimmune myocarditis-positive animals. Its resolution was sufficient to identify even small inflammatory foci, that is, at the surface of the right ventricle. Postmortem immunohistochemistry and flow cytometry confirmed the presence of perfluorocarbon in macrophages, dendritic cells, and granulocytes, but not in lymphocytes. The myocardial volume of elevated (19)F signal (rs=0.96; P<0.001), the (19)F signal-to-noise ratio (rs=0.92; P<0.001), and the (19)F signal integral (rs=0.96; P<0.001) at day 21 correlated with the histological myocarditis severity score.
CONCLUSIONS: In vivo (19)F-CMR was successfully used to visualize the inflammation specifically and robustly in experimental autoimmune myocarditis, and thus allowed for an unprecedented insight into the involvement of inflammatory cells in the disease process.
Mots-clé
Animals, Autoimmune Diseases/immunology, Autoimmune Diseases/metabolism, Dendritic Cells/immunology, Disease Models, Animal, Flow Cytometry, Fluorine/administration & dosage, Fluorine/diagnostic use, Fluorocarbons/administration & dosage, Fluorocarbons/diagnostic use, Granulocytes/immunology, Immunohistochemistry, Injections, Intravenous, Lymphocytes/immunology, Macrophages/immunology, Magnetic Resonance Imaging, Male, Mice, Mice, Inbred BALB C, Myeloid Cells/immunology, Myeloid Cells/metabolism, Myocarditis/immunology, Myocarditis/metabolism, Myocardium/immunology, Myocardium/metabolism, Predictive Value of Tests, Severity of Illness Index, Signal-To-Noise Ratio
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/08/2013 11:28
Dernière modification de la notice
20/08/2019 16:43
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