The DExH/D box protein HEL/UAP56 is essential for mRNA nuclear export in Drosophila.

Détails

ID Serval
serval:BIB_C7A2FDCD7E1B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The DExH/D box protein HEL/UAP56 is essential for mRNA nuclear export in Drosophila.
Périodique
Current Biology
Auteur(s)
Gatfield D., Le Hir H., Schmitt C., Braun I.C., Köcher T., Wilm M., Izaurralde E.
ISSN
0960-9822 (Print)
ISSN-L
0960-9822
Statut éditorial
Publié
Date de publication
2001
Volume
11
Numéro
21
Pages
1716-1721
Langue
anglais
Résumé
Dbp5 is the only member of the DExH/D box family of RNA helicases that is directly implicated in the export of messenger RNAs from the nucleus of yeast and vertebrate cells. Dbp5 localizes in the cytoplasm and at the cytoplasmic face of the nuclear pore complex (NPC). In an attempt to identify proteins present in a highly enriched NPC fraction, two other helicases were detected: RNA helicase A (RHA) and UAP56. This suggested a role for these proteins in nuclear transport. Contrary to expectation, we show that the Drosophila homolog of Dbp5 is not essential for mRNA export in cultured Schneider cells. In contrast, depletion of HEL, the Drosophila homolog of UAP56, inhibits growth and results in a robust accumulation of polyadenylated RNAs within the nucleus. Consequently, incorporation of [35S]methionine into newly synthesized proteins is inhibited. This inhibition affects the expression of both heat-shock and non-heat-shock mRNAs, as well as intron-containing and intronless mRNAs. In HeLa nuclear extracts, UAP56 preferentially, but not exclusively, associates with spliced mRNAs carrying the exon junction complex (EJC). We conclude that HEL is essential for the export of bulk mRNA in Drosophila. The association of human UAP56 with spliced mRNAs suggests that this protein might provide a functional link between splicing and export.
Mots-clé
Active Transport, Cell Nucleus, Adenosine Triphosphatases/genetics, Adenosine Triphosphatases/metabolism, Animals, Cell Nucleus/metabolism, Cells, Cultured, Cloning, Molecular, DNA, Complementary, Drosophila Proteins/metabolism, Drosophila melanogaster, HeLa Cells, Heat-Shock Response, Humans, Protein Biosynthesis, RNA Helicases/genetics, RNA Helicases/metabolism, RNA Splicing, RNA, Antisense, RNA, Messenger/metabolism, RNA, Small Interfering
Pubmed
Web of science
Open Access
Oui
Création de la notice
12/12/2012 12:22
Dernière modification de la notice
09/05/2019 1:06
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