The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing.

Détails

Ressource 1Télécharger: pmid27184842.pdf (11361.05 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_C76FB9B4613C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing.
Périodique
Cell reports
Auteur(s)
Degrauwe N., Schlumpf T.B., Janiszewska M., Martin P., Cauderay A., Provero P., Riggi N., Suvà M.L., Paro R., Stamenkovic I.
ISSN
2211-1247 (Electronic)
Statut éditorial
Publié
Date de publication
24/05/2016
Peer-reviewed
Oui
Volume
15
Numéro
8
Pages
1634-1647
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Cancer stem cells (CSCs) can drive tumor growth, and their maintenance may rely on post-transcriptional regulation of gene expression, including that mediated by microRNAs (miRNAs). The let-7 miRNA family has been shown to induce differentiation by silencing stem cell programs. Let-7-mediated target gene suppression is prevented by LIN28A/B, which reduce let-7 biogenesis in normal embryonic and some cancer stem cells and ensure maintenance of stemness. Here, we find that glioblastoma stem cells (GSCs) lack LIN28 and express both let-7 and their target genes, suggesting LIN28-independent protection from let-7 silencing. Using photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP), we show that insulin-like growth factor 2 mRNA-binding protein 2 (IMP2) binds to let-7 miRNA recognition elements (MREs) and prevents let-7 target gene silencing. Our observations define the RNA-binding repertoire of IMP2 and identify a mechanism whereby it supports GSC and neural stem cell specification.

Mots-clé
Base Sequence, Brain Neoplasms/genetics, Brain Neoplasms/pathology, Cell Adhesion, Gene Expression Regulation, Neoplastic, Gene Silencing, Glioblastoma/genetics, Glioblastoma/pathology, Humans, MicroRNAs/genetics, MicroRNAs/metabolism, Neoplastic Stem Cells/metabolism, Neoplastic Stem Cells/pathology, Neural Stem Cells/metabolism, Protein Binding, RNA, Messenger/genetics, RNA, Messenger/metabolism, RNA-Binding Proteins/metabolism, Spheroids, Cellular/pathology
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/05/2016 8:40
Dernière modification de la notice
09/05/2019 1:06
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