Proteomics application exercise of the Swiss Proteomics Society: report of the SPS'02 session.

Détails

ID Serval
serval:BIB_C74D1B8FE309
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Proteomics application exercise of the Swiss Proteomics Society: report of the SPS'02 session.
Périodique
Proteomics
Auteur(s)
Binz P.A., Abdi F., Affolter M., Allard L., Barblan J., Bhardwaj S., Bienvenut W.V., Bulet P., Burgess J., Carrette O., Corthals G., Delalande F., Diemer H., Favreau P., Giuliano E., Gueguen Y., Guillaume E., Hahner S., Man P., Michalet S., Neri D., Noukakis D., Palagi P., Paroutaud P., Pimenta D.C., Quadroni M., Resemann A., Richert S., Rybak J., Sanchez J.C., Scherl A., Scheurer S., Schweiger Hufnagel U., Siethoff C., Suckau D., van Dorsselaer A., Wagner Redeker W., Walter N., Stöcklin R.
ISSN
1615-9853 (Print)
ISSN-L
1615-9853
Statut éditorial
Publié
Date de publication
2003
Peer-reviewed
Oui
Volume
3
Numéro
8
Pages
1562-1566
Langue
anglais
Résumé
After the success of the mass spectrometry (MS) round table that was held at the first Swiss Proteomics Society congress (SPS'01) in Geneva, the SPS has organized a proteomics application exercise and allocated a full session at the SPS'02 congress. The main objective was to encourage the exchange of expertise in protein identification, with a focus on the use of mass spectrometry, and to create a bridge between the users' questions and the instrument providers' solutions. Two samples were sent to fifteen interested labs, including academic groups and MS hardware providers. Participants were asked to identify and partially characterize the samples. They consisted of a complex mixture of peptide/proteins (sample A) and an almost pure recombinant peptide carrying post-translational modifications (sample B). Sample A was an extract of snake venom from the species Bothrops jararaca. Sample B was a recombinant and modified peptide derived from the shrimp Penaeus vannamei penaeidin 3a. The eight labs that returned results reported the use of a wide range of MS instrumentation and techniques. They mentioned a variety of time and manpower allocations. The origin of sample A was generally identified together with a number of database protein entries. The difficulty of the sample identification lay in the incomplete knowledge of the Bothrops species genome sequence and is discussed. Sample B was generally and correctly identified as penaeidin. However, only one group reported the full primary structure. Interestingly, the approaches were again varied and are discussed in the text.
Mots-clé
Mass Spectrometry, Proteomics
Pubmed
Web of science
Création de la notice
24/01/2008 16:46
Dernière modification de la notice
03/03/2018 21:19
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