In vitro characterization of immune-related properties of human fetal bone cells for potential tissue engineering applications.

Details

Serval ID
serval:BIB_C6EEC596AC19
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In vitro characterization of immune-related properties of human fetal bone cells for potential tissue engineering applications.
Journal
Tissue Engineering. Part A
Author(s)
Montjovent M.O., Bocelli-Tyndall C., Scaletta C., Scherberich A., Mark S., Martin I., Applegate L.A., Pioletti D.P.
ISSN
1937-335X[electronic]
Publication state
Published
Issued date
2009
Volume
15
Number
7
Pages
1523-1532
Language
english
Abstract
We describe herein some immunological properties of human fetal bone cells recently tested for bone tissue-engineering applications. Adult mesenchymal stem cells (MSCs) and osteoblasts were included in the study for comparison. Surface markers involved in bone metabolism and immune recognition were analyzed using flow cytometry before and after differentiation or treatment with cytokines. Immunomodulatory properties were studied on activated peripheral blood mononuclear cells (PBMCs). The immuno-profile of fetal bone cells was further investigated at the gene expression level. Fetal bone cells and adult MSCs were positive for Stro-1, alkaline phosphatase, CD10, CD44, CD54, and beta2-microglobulin, but human leukocyte antigen (HLA)-I and CD80 were less present than on adult osteoblasts. All cells were negative for HLA-II. Treatment with recombinant human interferon gamma increased the presence of HLA-I in adult cells much more than in fetal cells. In the presence of activated PBMCs, fetal cells had antiproliferative effects, although with patterns not always comparable with those of adult MSCs and osteoblasts. Because of the immunological profile, and with their more-differentiated phenotype than of stem cells, fetal bone cells present an interesting potential for allogeneic cell source in tissue-engineering applications.
Keywords
Adult, Aged, Antibodies, Monoclonal/immunology, Antigens, CD28/metabolism, Antigens, CD3/metabolism, Bone and Bones/cytology, Bone and Bones/drug effects, Cell Proliferation/drug effects, Fetus/cytology, Fetus/drug effects, Flow Cytometry, Gene Expression Regulation/drug effects, Histocompatibility Antigens Class I/immunology, Histocompatibility Antigens Class II/immunology, Humans, Immunologic Factors/pharmacology, Intercellular Adhesion Molecule-1/immunology, Interferon-gamma, Recombinant/pharmacology, Leukocytes, Mononuclear/cytology, Leukocytes, Mononuclear/drug effects, Male, Middle Aged, Tissue Engineering/methods, Tumor Necrosis Factor-alpha/pharmacology
Pubmed
Web of science
Create date
09/02/2010 13:46
Last modification date
20/08/2019 15:42
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