The G0/G1 switch gene 2 is a novel PPAR target gene.

Détails

ID Serval
serval:BIB_C6EBFB10FA90
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The G0/G1 switch gene 2 is a novel PPAR target gene.
Périodique
Biochemical Journal
Auteur(s)
Zandbergen F., Mandard S., Escher P., Tan N.S., Patsouris D., Jatkoe T., Rojas-Caro S., Madore S., Wahli W., Tafuri S., Müller M., Kersten S.
ISSN
1470-8728[electronic], 0264-6021[linking]
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
392
Numéro
Pt 2
Pages
313-324
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
PPARs (peroxisome-proliferator-activated receptors) alpha, beta/delta and gamma are a group of transcription factors that are involved in numerous processes, including lipid metabolism and adipogenesis. By comparing liver mRNAs of wild-type and PPARalpha-null mice using microarrays, a novel putative target gene of PPARalpha, G0S2 (G0/G1 switch gene 2), was identified. Hepatic expression of G0S2 was up-regulated by fasting and by the PPARalpha agonist Wy14643 in a PPARalpha-dependent manner. Surprisingly, the G0S2 mRNA level was highest in brown and white adipose tissue and was greatly up-regulated during mouse 3T3-L1 and human SGBS (Simpson-Golabi-Behmel syndrome) adipogenesis. Transactivation, gel shift and chromatin immunoprecipitation assays indicated that G0S2 is a direct PPARgamma and probable PPARalpha target gene with a functional PPRE (PPAR-responsive element) in its promoter. Up-regulation of G0S2 mRNA seemed to be specific for adipogenesis, and was not observed during osteogenesis or myogenesis. In 3T3-L1 fibroblasts, expression of G0S2 was associated with growth arrest, which is required for 3T3-L1 adipogenesis. Together, these data indicate that G0S2 is a novel target gene of PPARs that may be involved in adipocyte differentiation.
Mots-clé
Adipocytes/cytology, Adipocytes/metabolism, Adipogenesis, Animals, Base Sequence, Cell Cycle Proteins/genetics, Cell Cycle Proteins/metabolism, Cell Line, Endoplasmic Reticulum/metabolism, Gene Deletion, Hepatocytes/cytology, Hepatocytes/metabolism, Humans, Liver/cytology, Male, Mice, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, PPAR alpha/genetics, PPAR alpha/metabolism, Promoter Regions, Genetic/genetics, Protein Transport, RNA, Messenger/genetics, RNA, Messenger/metabolism, Rats, Response Elements/genetics, Sequence Homology, Nucleic Acid, Substrate Specificity, Up-Regulation
Pubmed
Web of science
Création de la notice
24/01/2008 17:04
Dernière modification de la notice
03/03/2018 21:18
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