ATP citrate lyase activity is post-translationally regulated by sink strength and impacts the wax, cutin and rubber biosynthetic pathways.

Détails

ID Serval
serval:BIB_C6EABB90B9C7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
ATP citrate lyase activity is post-translationally regulated by sink strength and impacts the wax, cutin and rubber biosynthetic pathways.
Périodique
Plant Journal
Auteur(s)
Xing S., van Deenen N., Magliano P., Frahm L., Forestier E., Nawrath C., Schaller H., Gronover C.S., Prüfer D., Poirier Y.
ISSN
1365-313X (Electronic)
ISSN-L
0960-7412
Statut éditorial
Publié
Date de publication
2014
Volume
79
Numéro
2
Pages
270-284
Langue
anglais
Résumé
Cytosolic acetyl-CoA is involved in the synthesis of a variety of compounds, including waxes, sterols and rubber, and is generated by the ATP citrate lyase (ACL). Plants over-expressing ACL were generated in an effort to understand the contribution of ACL activity to the carbon flux of acetyl-CoA to metabolic pathways occurring in the cytosol. Transgenic Arabidopsis plants synthesizing the polyester polyhydroxybutyrate (PHB) from cytosolic acetyl-CoA have reduced growth and wax content, consistent with a reduction in the availability of cytosolic acetyl-CoA to endogenous pathways. Increasing the ACL activity via the over-expression of the ACLA and ACLB subunits reversed the phenotypes associated with PHB synthesis while maintaining polymer synthesis. PHB production by itself was associated with an increase in ACL activity that occurred in the absence of changes in steady-state mRNA or protein level, indicating a post-translational regulation of ACL activity in response to sink strength. Over-expression of ACL in Arabidopsis was associated with a 30% increase in wax on stems, while over-expression of a chimeric homomeric ACL in the laticifer of roots of dandelion led to a four- and two-fold increase in rubber and triterpene content, respectively. Synthesis of PHB and over-expression of ACL also changed the amount of the cutin monomer octadecadien-1,18-dioic acid, revealing an unsuspected link between cytosolic acetyl-CoA and cutin biosynthesis. Together, these results reveal the complexity of ACL regulation and its central role in influencing the carbon flux to metabolic pathways using cytosolic acetyl-CoA, including wax and polyisoprenoids.
Mots-clé
ATP citrate lyase, acetyl-CoA, cutin, rubber, Arabidopsis thaliana, wax, polyhydroxybutyrate, Taraxacum brevicorniculatum
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/08/2014 8:55
Dernière modification de la notice
20/08/2019 15:42
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