A role for the alpha-chain connecting peptide motif in mediating TCR-CD8 cooperation.

Détails

ID Serval
serval:BIB_C672291A6382
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A role for the alpha-chain connecting peptide motif in mediating TCR-CD8 cooperation.
Périodique
Journal of immunology
Auteur(s)
Naeher D., Luescher I.F., Palmer E.
ISSN
0022-1767
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
169
Numéro
6
Pages
2964-2970
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
To generate peripheral T cells that are both self-MHC restricted and self-MHC tolerant, thymocytes are subjected to positive and negative selection. How the TCR discriminates between positive and negative selection ligands is not well understood, although there is substantial evidence that the CD4 and CD8 coreceptors play an important role in this cell fate decision. We have previously identified an evolutionarily conserved motif in the TCR, the alpha-chain connecting peptide motif (alpha-CPM), which allows the TCR to deliver positive selection signals. Thymocytes expressing alpha-CPM-deficient receptors do not undergo positive selection, whereas their negative selection is not impaired. In this work we studied the ligand binding and receptor function of alpha-CPM-deficient TCRs by generating T cell hybridomas expressing wild-type or alpha-CPM-deficient forms of the T1 TCR. This K(d)-restricted TCR is specific for a photoreactive derivative of the Plasmodium berghei circumsporozoite peptide(252-260) IASA-YIPSAEK(ABA)I and is therefore amenable to TCR photoaffinity labeling. The experiments presented in this work show that alpha-CPM-deficient TCRs fail to cooperate with CD8 to enhance ligand binding and functional responses.
Mots-clé
Amino Acid Motifs/genetics, Amino Acid Sequence, Animals, Antigens, CD8/metabolism, Antigens, CD8/physiology, CD8-Positive T-Lymphocytes/immunology, CD8-Positive T-Lymphocytes/metabolism, Cell Line, Gene Expression Regulation/immunology, Genes, T-Cell Receptor alpha/genetics, Genes, T-Cell Receptor alpha/physiology, Humans, Hybridomas, Interleukin-2/biosynthesis, Ligands, Mice, Molecular Sequence Data, Peptide Fragments/genetics, Peptide Fragments/physiology, Protein Binding/genetics, Protein Binding/immunology, Receptors, Antigen, T-Cell, alpha-beta/biosynthesis, Receptors, Antigen, T-Cell, alpha-beta/genetics, Recombinant Fusion Proteins/biosynthesis, Recombinant Fusion Proteins/genetics
Pubmed
Web of science
Création de la notice
28/01/2008 12:19
Dernière modification de la notice
03/03/2018 21:17
Données d'usage