Selected contribution: limiting Na(+) transport rate in airway epithelia from alpha-ENaC transgenic mice: a model for pulmonary edema

Détails

ID Serval
serval:BIB_C60B661CC951
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Selected contribution: limiting Na(+) transport rate in airway epithelia from alpha-ENaC transgenic mice: a model for pulmonary edema
Périodique
Journal of Applied Physiology
Auteur(s)
Olivier R., Scherrer U., Horisberger J. D., Rossier B. C., Hummler E.
ISSN
8750-7587 (Print)
Statut éditorial
Publié
Date de publication
11/2002
Volume
93
Numéro
5
Pages
1881-87
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Nov
Résumé
The amiloride-sensitive epithelial Na(+) channel (ENaC) is essential for fluid clearance from the airways. An experimental animal model with a reduced expression of ENaC, the alpha-ENaC transgenic rescue mouse, is prone to develop edema under hypoxia exposure. This strongly suggests an involvement of ENaC in the pathogenesis of pulmonary edema. To investigate the pathogenesis of this type of edema, primary cultures of tracheal cells from these mice were studied in vitro. An ~60% reduction in baseline amiloride-sensitive Na(+) transport was observed, but the pharmacological characteristics and physiological regulation of the channel were similar to those observed in cells from wild-type mice. Aprotinin, an inhibitor of serine proteases, blocked 50-60% of the basal transepithelial current, hypoxia induced downregulation of Na(+) transport, and beta-adrenergic stimulation was effective to stimulate Na(+) transport after the hypoxia-induced decrease. When downregulation of ENaC activity (such as observed under hypoxia) is added to a low "constitutive" ENaC expression, the resulting reduced Na(+) transport rate may be insufficient for airway fluid clearance and favor pulmonary edema.
Mots-clé
Amiloride/pharmacology Animals Anoxia/metabolism Aprotinin/pharmacology Biological Transport/drug effects Cells, Cultured Epithelial Cells/metabolism Epithelial Sodium Channel Mice Mice, Transgenic/genetics Pulmonary Edema/*metabolism Reference Values Serine Proteinase Inhibitors/pharmacology Sodium/*metabolism Sodium Channels/genetics/*metabolism Terbutaline/pharmacology Trachea/cytology/*metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 13:37
Dernière modification de la notice
03/03/2018 21:16
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