Metabolic response to various beta-adrenoceptor agonists in beta3-adrenoceptor knockout mice: evidence for a new beta-adrenergic receptor in brown adipose tissue.

Détails

ID Serval
serval:BIB_C5ADCB6D80B1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Metabolic response to various beta-adrenoceptor agonists in beta3-adrenoceptor knockout mice: evidence for a new beta-adrenergic receptor in brown adipose tissue.
Périodique
British Journal of Pharmacology
Auteur(s)
Preitner F., Muzzin P., Revelli J.P., Seydoux J., Galitzky J., Berlan M., Lafontan M., Giacobino J.P.
ISSN
0007-1188 (Print)
ISSN-L
0007-1188
Statut éditorial
Publié
Date de publication
1998
Volume
124
Numéro
8
Pages
1684-1688
Langue
anglais
Résumé
The beta3-adrenoceptor plays an important role in the adrenergic response of brown and white adipose tissues (BAT and WAT). In this study, in vitro metabolic responses to beta-adrenoceptor stimulation were compared in adipose tissues of beta3-adrenoceptor knockout and wild type mice. The measured parameters were BAT fragment oxygen uptake (MO2) and isolated white adipocyte lipolysis. In BAT of wild type mice (-)-norepinephrine maximally stimulated MO2 4.1+/-0.8 fold. Similar maximal stimulations were obtained with beta1-, beta2- or beta3-adrenoceptor selective agonists (dobutamine 5.1+/-0.3, terbutaline 5.3+/-0.3 and CL 316,243 4.8+/-0.9 fold, respectively); in BAT of beta3-adrenoceptor knockout mice, the beta1- and beta2-responses were fully conserved. In BAT of wild type mice, the beta1/beta2-antagonist and beta3-partial agonist CGP 12177 elicited a maximal MO2 response (4.7+/-0.4 fold). In beta3-adrenoceptor knockout BAT, this response was fully conserved despite an absence of response to CL 316,243. This unexpected result suggests that an atypical beta-adrenoceptor, distinct from the beta1-, beta2- and beta3-subtypes and referred to as a putative beta4-adrenoceptor is present in BAT and that it can mediate in vitro a maximal MO2 stimulation. In isolated white adipocytes of wild type mice, (-)-epinephrine maximally stimulated lipolysis 12.1+/-2.6 fold. Similar maximal stimulations were obtained with beta1-, beta2- or beta3-adrenoceptor selective agonists (TO509 12+/-2, procaterol 11+/-3, CL 316,243 11+/-3 fold, respectively) or with CGP 12177 (7.1+/-1.5 fold). In isolated white adipocytes of beta3-adrenoceptor knockout mice, the lipolytic responses to (-)epinephrine, to the beta1-, beta2-, beta3-adrenoceptor selective agonists and to CGP 12177 were almost or totally depressed, whereas those to ACTH, forskolin and dibutyryl cyclic AMP were conserved.
Mots-clé
Adipose Tissue/cytology, Adipose Tissue/drug effects, Adipose Tissue, Brown/cytology, Adipose Tissue, Brown/drug effects, Adrenergic beta-Agonists/pharmacology, Animals, Epinephrine/metabolism, Glycerol/metabolism, Lipolysis/drug effects, Male, Mice, Mice, Knockout, Oxygen Consumption/drug effects, Propanolamines/pharmacology, Receptors, Adrenergic, beta/genetics, Receptors, Adrenergic, beta/physiology, Receptors, Adrenergic, beta-3
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/12/2012 16:50
Dernière modification de la notice
09/05/2019 1:00
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