The long-term survival of in vitro engineered nervous tissue derived from the specific neural differentiation of mouse embryonic stem cells.

Détails

ID Serval
serval:BIB_C5730A73B1B8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The long-term survival of in vitro engineered nervous tissue derived from the specific neural differentiation of mouse embryonic stem cells.
Périodique
Biomaterials
Auteur(s)
Dubois-Dauphin M.L., Toni N., Julien S.D., Charvet I., Sundstrom L.E., Stoppini L.
ISSN
1878-5905[electronic], 0142-9612[linking]
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
31
Numéro
27
Pages
7032-7042
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
Embryonic stem cells (ESCs) offer attractive prospective as potential source of neurons for cell replacement therapy in human neurodegenerative diseases. Besides, ESCs neural differentiation enables in vitro tissue engineering for fundamental research and drug discovery aimed at the nervous system. We have established stable and long-term three-dimensional (3D) culture conditions which can be used to model long latency and complex neurodegenerative diseases. Mouse ESCs-derived neural progenitor cells generated by MS5 stromal cells induction, result in strictly neural 3D cultures of about 120-mum thick, whose cells expressed mature neuronal, astrocytes and myelin markers. Neurons were from the glutamatergic and gabaergic lineages. This nervous tissue was spatially organized in specific layers resembling brain sub-ependymal (SE) nervous tissue, and was maintained in vitro for at least 3.5 months with great stability. Electron microscopy showed the presence of mature synapses and myelinated axons, suggesting functional maturation. Electrophysiological activity revealed biological signals involving action potential propagation along neuronal fibres and synaptic-like release of neurotransmitters. The rapid development and stabilization of this 3D cultures model result in an abundant and long-lasting production that is compatible with multiple and productive investigations for neurodegenerative diseases modeling, drug and toxicology screening, stress and aging research.
Mots-clé
Embryonic stem cells, neural cells, tissue engineering, brain, pharmacology
Pubmed
Web of science
Création de la notice
21/07/2010 14:25
Dernière modification de la notice
03/03/2018 21:15
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