Gene expression profiling of neural stem cells and their neuronal progeny reveals IGF2 as a regulator of adult hippocampal neurogenesis.

Détails

Ressource 1Télécharger: Bracko et al 2012.pdf (2748.83 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_C541310F3920
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Gene expression profiling of neural stem cells and their neuronal progeny reveals IGF2 as a regulator of adult hippocampal neurogenesis.
Périodique
The Journal of neuroscience
Auteur(s)
Bracko O., Singer T., Aigner S., Knobloch M., Winner B., Ray J., Clemenson G.D., Suh H., Couillard-Despres S., Aigner L., Gage F.H., Jessberger S.
ISSN
1529-2401 (Electronic)
ISSN-L
0270-6474
Statut éditorial
Publié
Date de publication
07/03/2012
Peer-reviewed
Oui
Volume
32
Numéro
10
Pages
3376-3387
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Neural stem cells (NSCs) generate neurons throughout life in the hippocampal dentate gyrus (DG). How gene expression signatures differ among NSCs and immature neurons remains largely unknown. We isolated NSCs and their progeny in the adult DG using transgenic mice expressing a GFP reporter under the control of the Sox2 promoter (labeling NSCs) and transgenic mice expressing a DsRed reporter under the control of the doublecortin (DCX) promoter (labeling immature neurons). Transcriptome analyses revealed distinct gene expression profiles between NSCs and immature neurons. Among the genes that were expressed at significantly higher levels in DG NSCs than in immature neurons was the growth factor insulin-like growth factor 2 (IGF2). We show that IGF2 selectively controls proliferation of DG NSCs in vitro and in vivo through AKT-dependent signaling. Thus, by gene expression profiling of NSCs and their progeny, we have identified IGF2 as a novel regulator of adult neurogenesis.
Mots-clé
Adult Stem Cells/cytology, Adult Stem Cells/physiology, Animals, Cell Differentiation/genetics, Cells, Cultured, Female, Gene Expression Profiling/methods, Hippocampus/cytology, Hippocampus/physiology, Insulin-Like Growth Factor II/physiology, Mice, Mice, Inbred C57BL, Mice, Transgenic, Neural Stem Cells/cytology, Neural Stem Cells/physiology, Neurogenesis/genetics, Neurons/cytology, Neurons/physiology, Transcriptome/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/05/2018 9:34
Dernière modification de la notice
20/08/2019 16:40
Données d'usage