Non-contact microdrilling of mouse zona pellucida with an objective-delivered 1.48-microns diode laser.
Details
Serval ID
serval:BIB_C51138ACA262
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Non-contact microdrilling of mouse zona pellucida with an objective-delivered 1.48-microns diode laser.
Journal
Lasers in surgery and medicine
ISSN
0196-8092
Publication state
Published
Issued date
1996
Peer-reviewed
Oui
Volume
18
Number
1
Pages
52-62
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
BACKGROUND and OBJECTIVE: A non-touch laser-induced microdrilling procedure is studied on mouse zona pellucida (ZP). STUDY DESIGN/MATERIALS and METHODS: A 1.48-microns diode laser beam is focused in a 8-microns spot through a 45x objective of an inverted microscope. Mouse zygotes, suspended in a culture medium, are microdrilled by exposing their ZP to a short laser irradiation and allowed to develop in vitro. RESULTS: Various sharp-edged holes can be generated in the ZP with a single laser irradiation. Sizes can be varied by changing irradiation time (3-100 ms) or laser power (22-55 mW). Drilled zygotes present no signs of thermal damage under light and scanning electron microscopy and develop as expected in vitro, except for a distinct eight-shaped hatching behavior. CONCLUSION: The microdrilling procedure can generate standardized holes in mouse ZP, without any visible side effects. The hole formation can be explained by a local photothermolysis of the protein matrix.
Keywords
Animals, Blastocyst, Culture Media, Culture Techniques, Extracellular Matrix Proteins, Hot Temperature, Laser Therapy, Lasers, Lenses, Mice, Mice, Inbred Strains, Microscopy, Microscopy, Electron, Scanning, Microsurgery, Zona Pellucida, Zygote
Pubmed
Web of science
Create date
25/01/2008 16:40
Last modification date
20/08/2019 16:40
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