Calcium Microdomains Control Exo-Endocytosis of Synaptic-Like Microvesicles in Astrocytes

Détails

ID Serval
serval:BIB_C4FFE83230D6
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Abstract (résumé de présentation): article court qui reprend les éléments essentiels présentés à l'occasion d'une conférence scientifique dans un poster ou lors d'une intervention orale.
Collection
Publications
Titre
Calcium Microdomains Control Exo-Endocytosis of Synaptic-Like Microvesicles in Astrocytes
Titre de la conférence
9th European Meeting on Glial Cells in Health and Disease
Auteur(s)
Marchaland J., Cali C., Voglmaier S.M., Li H., Regazzi R., Edwards R.H., Bezzi P.
Adresse
Paris, France, September 08-12, 2009
ISBN
0894-1491
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
57
Série
Glia
Pages
45
Langue
anglais
Notes
Publication type : Meeting Abstract
Résumé
During the last decade, the discovery that astrocytes possess a nonelectrical form of excitability (Ca21-excitability) that leads to the release of chemical transmitters, an activity called ''gliotransmission'', indicates that these cells may have additional important roles in brain function. Elucidating the stimulus-secretion coupling leading to the exocytic release of chemical transmitters (such as glutamate, Bezzi et al., Nature Neurosci, 2004) may therefore clarify i) whether astrocytes represent in full a new class of secretory cells in the brain and ii) whether they can participate to the fast brain signaling in the brain. Here by using a recently developed approach for studying vesicle recycling dynamics at synapses (Voglmaier et al., Neuron, 2006; Balaji and Ryan, PNAS, 2007) combined with epifluorescence and total internal reflection fluorescence (TIRF) imaging, we investigated the spatiotemporal characteristics of stimulus-secretion coupling leading glutamate exocytosis of synaptic-like microvesicles (SLMVs) in astrocytes. We performed the analysis at both the whole-cell and single-vesicle levels providing the first system for comparing exo-endocytic processes in astrocytes with those in neurons. Both the time course and modalities of secretion in astrocytes present more similarities to neurons then previously expected. We found that 1. the G-protein-coupled receptor (GPCR)-evoked exocytosis reached the maximum on a ms time scale and that 2. ER tubuli formed sub-micrometer domains beneath the plasma membrane in close proximity to exocytic vesicles, where fusion events were spatiotemporally correlated with fast Ca21 events.
Web of science
Création de la notice
04/12/2009 9:54
Dernière modification de la notice
20/08/2019 16:40
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