Cloning and characterization of SNAP50, a subunit of the snRNA-activating protein complex SNAPc.

Détails

ID Serval
serval:BIB_C4ECDAAEBF03
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cloning and characterization of SNAP50, a subunit of the snRNA-activating protein complex SNAPc.
Périodique
EMBO Journal
Auteur(s)
Henry R.W., Ma B., Sadowski C.L., Kobayashi R., Hernandez N.
ISSN
0261-4189
Statut éditorial
Publié
Date de publication
12/1996
Peer-reviewed
Oui
Volume
15
Numéro
24
Pages
7129-7136
Langue
anglais
Résumé
The human RNA polymerase II and III snRNA promoters share a common basal element, the proximal sequence element (PSE), which is recognized by a complex we refer to as the snRNA-activating protein complex (SNAPc). Biochemical purifications suggest that SNAPc is composed of at least four polypeptides of 43, 45, 50 and 190 kDa, as well as variable amounts of the TATA box binding protein, TBP. cDNAs encoding the 43 and 45 kDa subunits, SNAP43 and SNAP45, have been isolated, but there is no evidence that either of these subunits contacts DNA. Here we report the isolation of cDNAs encoding the 50 kDa subunit of SNAPc, SNAP50. The open reading frame predicts a 411 amino acid protein, which contains two potential zinc finger motifs. Depletions with anti-SNAP50 antibodies inhibit RNA polymerase II and III snRNA gene transcription in vitro. SNAP50 interacts with SNAP43 in co-immunoprecipitation experiments, but not with SNAP45 or TBP. UV cross-linking experiments suggest that SNAP50 contacts DNA in the SNAP complex. These results are consistent with the same core SNAP complex recognizing the PSEs of RNA polymerase II and III snRNA promoters, and provide an initial view of the architecture of the SNAP complex.
Mots-clé
Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Complementary, DNA-Binding Proteins, Humans, Molecular Sequence Data, Protein Binding, Proteins, RNA Polymerase II, RNA Polymerase III, RNA, Small Nuclear, RNA-Binding Proteins, Transcription Factors, Transcription, Genetic
Pubmed
Web of science
Création de la notice
21/01/2008 17:33
Dernière modification de la notice
03/03/2018 21:14
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