Relative importance of CD4+ and CD8+ T cell repertoires in the development of acute graft-versus-host disease in a murine model of bone marrow transplantation

Details

Serval ID
serval:BIB_C4E0BE7D7712
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Relative importance of CD4+ and CD8+ T cell repertoires in the development of acute graft-versus-host disease in a murine model of bone marrow transplantation
Journal
Bone Marrow Transplantation
Author(s)
Miconnet  I., de La Selle  V., Bruley-Rosset  M.
ISSN
0268-3369 (Print)
Publication state
Published
Issued date
03/1998
Volume
21
Number
6
Pages
583-90
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Abstract
T cell repertoire alterations occurring after allogeneic BMT and related emergence of aGVHD has not been directly demonstrated. CD4, CD8 and Vbeta usage of T cells infiltrating spleen, lymph nodes and liver was compared in lethally irradiated F1(DBA/2 x B10.D2) recipients which develop (GVHD mice) or not (long survivor:LS mice) aGVHD across minor histocompatibility antigens (mHAgs) and Mtv-6 and Mtv-7 encoded super-antigens (SAgs) barriers according to experimental conditions. The early expansion in GVHD mice of CD4Vbeta6+ and of CD4Vbeta3+ T cell subsets specific for Mtv-7 and Mtv-6 SAgs, respectively, is abolished in LS protected mice. By contrast, CD8+ T cells infiltrate lymph nodes, the liver but not the spleen of LS as in GVHD mice. Vbeta subset overexpression is frequent in all T cell phenotypes in GVHD but only among CD8+ T cells in LS mice. Predominant Vbeta pattern subpopulation is unique to each mouse. Overexpressed Vbeta subpopulation sequencing clearly indicates that expansion results from a very limited number of clones. Association of a given Vbeta segment with different Jbeta for each mouse suggests that the response is directed towards many different antigens. The data emphasize that Mtv-SAg and mHAgs CD4+ T cells are of crucial importance during GVHD and that there is no relationship between CD8+ T cell repertoires and pathological status.
Keywords
Animals Antigens, Surface/immunology Antigens, Viral/immunology *Bone Marrow Transplantation CD4-Positive T-Lymphocytes/*immunology CD8-Positive T-Lymphocytes/*immunology Disease Models, Animal Graft vs Host Disease/*immunology/pathology Liver/immunology/pathology Lymph Nodes/immunology/pathology Mammary Tumor Virus, Mouse/immunology Mice Mice, Inbred DBA Minor Histocompatibility Antigens/immunology Receptors, Antigen, T-Cell, alpha-beta/immunology
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 15:08
Last modification date
20/08/2019 15:40
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