Casparian strip diffusion barrier in Arabidopsis is made of a lignin polymer without suberin.

Détails

ID Serval
serval:BIB_C4DF289D3D4F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Casparian strip diffusion barrier in Arabidopsis is made of a lignin polymer without suberin.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Naseer S., Lee Y., Lapierre C., Franke R., Nawrath C., Geldner N.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
2012
Volume
109
Numéro
25
Pages
10101-10106
Langue
anglais
Résumé
Casparian strips are ring-like cell-wall modifications in the root endodermis of vascular plants. Their presence generates a paracellular barrier, analogous to animal tight junctions, that is thought to be crucial for selective nutrient uptake, exclusion of pathogens, and many other processes. Despite their importance, the chemical nature of Casparian strips has remained a matter of debate, confounding further molecular analysis. Suberin, lignin, lignin-like polymers, or both, have been claimed to make up Casparian strips. Here we show that, in Arabidopsis, suberin is produced much too late to take part in Casparian strip formation. In addition, we have generated plants devoid of any detectable suberin, which still establish functional Casparian strips. In contrast, manipulating lignin biosynthesis abrogates Casparian strip formation. Finally, monolignol feeding and lignin-specific chemical analysis indicates the presence of archetypal lignin in Casparian strips. Our findings establish the chemical nature of the primary root-diffusion barrier in Arabidopsis and enable a mechanistic dissection of the formation of Casparian strips, which are an independent way of generating tight junctions in eukaryotes.
Mots-clé
Arabidopsis/physiology, Biopolymers/physiology, Lignin/physiology, Lipids/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/10/2012 10:52
Dernière modification de la notice
20/08/2019 15:40
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