Article: article from journal or magazin.
OmpA targets dendritic cells, induces their maturation and delivers antigen into the MHC class I presentation pathway
Journal Article --- Old month value: Dec
We analyzed the interaction between a bacterial cell wall protein and dendritic cells (DCs). Outer membrane protein A from Klebsiella pneumoniae (kpOmpA) specifically bound to professional antigen presenting cells and was endocytosed by immature DCs via a receptor-dependent mechanism. kpOmpA signaled through Toll-like receptor 2, induced DCs to produce interleukin 12 and induced maturation of DCs. Whole antigen that was coupled to kpOmpA and injected into mice was taken up by DCs and delivered to the conventional cytosolic MHC class I presentation pathway. kpOmpA also primed antigen-specific CD8+ CTLs in the absence of CD4+ T cell help or adjuvant and elicited therapeutic immunity to antigen-expressing tumors. Thus, OmpA belongs to a class of proteins that are able to elicit CTL responses to exogenous antigen.
Animals *Antigen Presentation Antigen-Presenting Cells/cytology/immunology Bacterial Outer Membrane Proteins/*immunology/*metabolism Cell Differentiation Cell Line Dendritic Cells/*cytology/*immunology *Drosophila Proteins Endocytosis Female Histocompatibility Antigens Class I/metabolism Humans Klebsiella pneumoniae/immunology Membrane Glycoproteins/metabolism Mice Mice, Inbred C57BL Mice, Knockout Neoplasms, Experimental/immunology/therapy Ovalbumin/immunology Receptors, Cell Surface/metabolism Signal Transduction T-Lymphocytes, Cytotoxic/immunology Toll-Like Receptor 2 Toll-Like Receptors
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