Clonal heterogeneity in the functional requirement for Lyt-2/3 molecules on cytolytic T lymphocytes: analysis by antibody blocking and selective trypsinization

Détails

ID Serval
serval:BIB_C4921140AD23
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Clonal heterogeneity in the functional requirement for Lyt-2/3 molecules on cytolytic T lymphocytes: analysis by antibody blocking and selective trypsinization
Périodique
Journal of Experimental Medicine
Auteur(s)
MacDonald  H. R., Glasebrook  A. L., Cerottini  J. C.
ISSN
0022-1007 (Print)
Statut éditorial
Publié
Date de publication
12/1982
Volume
156
Numéro
6
Pages
1711-22
Notes
Journal Article --- Old month value: Dec 1
Résumé
While it is well established that murine cytolytic T lymphocytes (CTL) express the Lyt-2/3 molecular complex on their surface, conflicting results have been reported concerning the role of this complex in CTL activity. In the present study this question was reinvestigated at the clonal level. Although different (H-2b anti-H-2d) CTL clones expressed comparable amounts of Lyt-2/3 molecules, as assessed by quantitative flow microfluorometry, the activity of some clones was inhibited by low doses (10 ng) of monoclonal anti-Lyt-2 or anti-Lyt-3 antibodies (in the absence of complement), whereas other clones were not inhibited by either antibody at doses as high as 5 microgram. Treatment of these clones with doses of trypsin sufficient to cleave Lyt-2/3 antigenic determinants from the cell surface resulted in a similar dissociation: clones that were inhibited by antibodies lost cytolytic activity, whereas "uninhibited" clones were unaffected by trypsin treatment. Moreover, the dissociation observed among different alloreactive clones could be demonstrated with self-H-2-restricted (H-2b anti-MSV) clones exhibiting cross-reactivity with normal H-2d products. The lytic activity of these clones against the relevant syngeneic target cells was unaffected by anti-Lyt-2 antibodies or trypsin, whereas their cross-reactivity on H-2d target cells was abolished by either treatment. These results provide direct evidence for clonal heterogeneity in the requirement for Lyt-2/3 molecules in CTL-mediated lysis. It is proposed that the function of Lyt-2/3 molecules is to stabilize the interaction between CTL receptors and the corresponding antigens on the target cells and that the requirement for such a stabilization is correlated with low number and/or affinity of CTL receptors.
Mots-clé
Animals Antibodies, Monoclonal/immunology Antigens, Heterophile/*immunology Antigens, Ly/analysis/*immunology Binding, Competitive Clone Cells/immunology Cross Reactions Cytotoxicity, Immunologic/drug effects Mice Mice, Inbred C57BL Mice, Inbred CBA Rats T-Lymphocytes, Cytotoxic/*immunology Trypsin/pharmacology
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:14
Dernière modification de la notice
20/08/2019 16:40
Données d'usage