Free ligand-induced dissociation of MHC-antigen complexes

Détails

ID Serval
serval:BIB_C46F6E740A3E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Free ligand-induced dissociation of MHC-antigen complexes
Périodique
Journal of Immunology
Auteur(s)
Pedrazzini  T., Sette  A., Albertson  M., Grey  H. M.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
05/1991
Volume
146
Numéro
10
Pages
3496-501
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: May 15
Résumé
We have analyzed the stability of Ag-class II complexes on the surface of live APC. It was found that the disappearance of complexes formed by I-Ed and the hen egg lysozyme 107-116 peptide is twice as rapid on the surface of live, as compared to glutaraldehyde-fixed APC. Moreover, the addition of peptides with a high affinity for I-Ed could reduce the half-life of Ag-class II complexes on either live or fixed APC. The same effect was detectable using purified class II molecules adhered on plastic microtiter wells, and even in the case of complexes formed in vitro between radiolabeled Ag and purified class II molecules. This effect of accelerated dissociation appeared to be specific because only I-Ed binding peptides were able to accelerate the dissociation of the hen egg lysozyme 107-116/I-Ed complex either on the surface of cells or in purified form in solution, and high affinity I-Ed binders did not affect the half-life of purified OVA 323-339/I-Ad complexes. These results demonstrate that free ligand can influence the kinetics of dissociation of class II-peptide complexes, and therefore suggest that a mechanism other than the classical first order kinetics may be involved in this process.
Mots-clé
Antigen-Presenting Cells/drug effects/*metabolism Binding Sites Histocompatibility Antigens Class II/*metabolism Humans Kinetics Ligands *Major Histocompatibility Complex Peptides/pharmacology
Pubmed
Web of science
Création de la notice
25/01/2008 9:45
Dernière modification de la notice
03/03/2018 21:13
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