Insulin-secreting beta-cell dysfunction induced by human lipoproteins.

Détails

ID Serval
serval:BIB_C41B6DC60022
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Insulin-secreting beta-cell dysfunction induced by human lipoproteins.
Périodique
Journal of Biological Chemistry
Auteur(s)
Roehrich M.E., Mooser V., Lenain V., Herz J., Nimpf J., Azhar S., Bideau M., Capponi A., Nicod P., Haefliger J.A., Waeber G.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
2003
Peer-reviewed
Oui
Volume
278
Numéro
20
Pages
18368-18375
Langue
anglais
Résumé
Diabetes is associated with significant changes in plasma concentrations of lipoproteins. We tested the hypothesis that lipoproteins modulate the function and survival of insulin-secreting cells. We first detected the presence of several receptors that participate in the binding and processing of plasma lipoproteins and confirmed the internalization of fluorescent low density lipoprotein (LDL) and high density lipoprotein (HDL) particles in insulin-secreting beta-cells. Purified human very low density lipoprotein (VLDL) and LDL particles reduced insulin mRNA levels and beta-cell proliferation and induced a dose-dependent increase in the rate of apoptosis. In mice lacking the LDL receptor, islets showed a dramatic decrease in LDL uptake and were partially resistant to apoptosis caused by LDL. VLDL-induced apoptosis of beta-cells involved caspase-3 cleavage and reduction in the levels of the c-Jun N-terminal kinase-interacting protein-1. In contrast, the proapoptotic signaling of lipoproteins was antagonized by HDL particles or by a small peptide inhibitor of c-Jun N-terminal kinase. The protective effects of HDL were mediated, in part, by inhibition of caspase-3 cleavage and activation of Akt/protein kinase B. In conclusion, human lipoproteins are critical regulators of beta-cell survival and may therefore contribute to the beta-cell dysfunction observed during the development of type 2 diabetes.
Mots-clé
Animals, Apoptosis, Blotting, Western, Caspase 3, Caspases/metabolism, Cell Division, Cell Survival, Cells, Cultured, DNA, Complementary/metabolism, Enzyme Activation, Humans, Immunohistochemistry, In Situ Nick-End Labeling, Insulin/metabolism, Islets of Langerhans/cytology, Islets of Langerhans/metabolism, Lipoproteins/metabolism, Lipoproteins, LDL/metabolism, Lipoproteins, VLDL/metabolism, Mice, Mice, Inbred C57BL, Protein Structure, Tertiary, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins/metabolism, Proto-Oncogene Proteins c-akt, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Time Factors
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 14:48
Dernière modification de la notice
20/08/2019 16:39
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