Improved cutaneous healing in diabetic mice exposed to healthy peripheral circulation.

Détails

ID Serval
serval:BIB_C4124A52393A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Improved cutaneous healing in diabetic mice exposed to healthy peripheral circulation.
Périodique
The Journal of investigative dermatology
Auteur(s)
Pietramaggiori G., Scherer S.S., Alperovich M., Chen B., Orgill D.P., Wagers A.J.
ISSN
1523-1747 (Electronic)
ISSN-L
0022-202X
Statut éditorial
Publié
Date de publication
09/2009
Peer-reviewed
Oui
Volume
129
Numéro
9
Pages
2265-2274
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Impaired repair of skin defects is a major complication of diabetes; yet, the pathophysiology of diabetic (db) wound healing remains largely opaque. Here, we investigate the role of humoral factors in modulating db wound repair by generating chimeric animals through parabiotic joining of wild-type (wt) and diabetic (db/db) mice. This strategy allows wounds on healing-deficient db/db mice to be exposed to factors derived from the wt circulation at physiologically appropriate concentrations. When compared with db controls, chimeric db/db animals showed significantly improved healing of full-thickness, cutaneous wounds, with enhanced granulation tissue formation, angiogenesis, cell proliferation, and collagen deposition. Glycemic control was unaffected by parabiosis; however, the distribution of circulating leukocytes, altered in db controls, normalized in db-chimeras. Both wt and db cells were recruited from circulation into db wounds, but wt cells never exceeded 20% of total cells. Improved angiogenesis persisted in db-chimeras separated 24 hours after wounding, suggesting the existence of long-term normalizing factors. This study establishes a new model for studying db wound healing, and shows a key role for circulating factors in normalizing wound repair in diabetes.

Mots-clé
Animals, Cell Proliferation, Collagen/metabolism, Diabetes Mellitus/blood, Diabetes Mellitus/physiopathology, Female, Granulation Tissue/physiopathology, Mice, Mice, Inbred C57BL, Neovascularization, Physiologic, Parabiosis, Wound Healing/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
16/01/2018 15:57
Dernière modification de la notice
09/05/2019 0:55
Données d'usage