Article: article from journal or magazin.
An NK-like cell limits the adoptive response from "pre-progenitor" B cells.
Australian Journal of Experimental Biology and Medical Science
Evidence is presented that a dividing, Thy 1 . 2 positive and NK 1 . 2 positive cell with a suppressive effect on adoptive responses is present in the spleens of normal and athymic mice, and that its function may be enhanced by non-specific antigenic stimulation. NK cells may thus exert a controlling function on the proliferation and differentiation of B cells. The main experimental observation is that under certain assay conditions incubation of spleen cells with 3H-TdR in order to kill dividing, non-specifically activated 'pre-progenitor' B cells may actually give a marked increase rather than a decrease in a subsequent hapten-specific primary adoptive response. An activated 'suppressor' population was proposed as the reason for this effect. This anomalous effect may be avoided and the cycle state of the B cells assessed by prior elimination of cells bearing Thy 1 antigen, together with the use of a more reliable hydroxyurea incubation to kill dividing B cells.
Animals, Antigens/administration & dosage, Antilymphocyte Serum/pharmacology, B-Lymphocytes/immunology, Cell Separation, Hematopoietic Stem Cells/immunology, Horses, Immunization, Passive, Killer Cells, Natural/immunology, Killer Cells, Natural/physiology, Lymphocyte Activation, Male, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, T-Lymphocytes, Regulatory/classification, T-Lymphocytes, Regulatory/immunology
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