Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite <i>Leishmania</i>.

Détails

Ressource 1Télécharger: Fasel29284754.pdf (2689.82 [Ko])
Etat: Serval
Version: Author's accepted manuscript
ID Serval
serval:BIB_C3DDB4066142
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite <i>Leishmania</i>.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Grybchuk D., Akopyants N.S., Kostygov A.Y., Konovalovas A., Lye L.F., Dobson D.E., Zangger H., Fasel N., Butenko A., Frolov A.O., Votýpka J., d'Avila-Levy C.M., Kulich P., Moravcová J., Plevka P., Rogozin I.B., Serva S., Lukeš J., Beverley S.M., Yurchenko V.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
16/01/2018
Peer-reviewed
Oui
Volume
115
Numéro
3
Pages
E506-E515
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, N.I.H., Intramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Knowledge of viral diversity is expanding greatly, but many lineages remain underexplored. We surveyed RNA viruses in 52 cultured monoxenous relatives of the human parasite <i>Leishmania</i> ( <i>Crithidia</i> and <i>Leptomonas</i> ), as well as plant-infecting <i>Phytomonas</i> <i>Leptomonas pyrrhocoris</i> was a hotbed for viral discovery, carrying a virus (Leptomonas pyrrhocoris ostravirus 1) with a highly divergent RNA-dependent RNA polymerase missed by conventional BLAST searches, an emergent clade of tombus-like viruses, and an example of viral endogenization. A deep-branching clade of trypanosomatid narnaviruses was found, notable as <i>Leptomonas seymouri</i> bearing Narna-like virus 1 (LepseyNLV1) have been reported in cultures recovered from patients with visceral leishmaniasis. A deep-branching trypanosomatid viral lineage showing strong affinities to bunyaviruses was termed " <i>Leishbunyavirus</i> " (LBV) and judged sufficiently distinct to warrant assignment within a proposed family termed " <i>Leishbunyaviridae</i> " Numerous relatives of trypanosomatid viruses were found in insect metatranscriptomic surveys, which likely arise from trypanosomatid microbiota. Despite extensive sampling we found no relatives of the totivirus <i>Leishmaniavirus</i> (LRV1/2), implying that it was acquired at about the same time the <i>Leishmania</i> became able to parasitize vertebrates. As viruses were found in over a quarter of isolates tested, many more are likely to be found in the >600 unsurveyed trypanosomatid species. Viral loss was occasionally observed in culture, providing potentially isogenic virus-free lines enabling studies probing the biological role of trypanosomatid viruses. These data shed important insights on the emergence of viruses within an important trypanosomatid clade relevant to human disease.
Mots-clé
Animals, Euglenozoa Infections/parasitology, Euglenozoa Infections/veterinary, Genetic Variation, Host Specificity, Host-Pathogen Interactions, Humans, Phylogeny, RNA Viruses/genetics, RNA Viruses/isolation & purification, Trypanosomatina/virology, Bunyavirales, Trypanosomatidae, coevolution, coinfection, persistent virus infection
Pubmed
Web of science
Open Access
Oui
Création de la notice
12/01/2018 9:10
Dernière modification de la notice
09/05/2019 0:54
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