Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy.

Details

Serval ID
serval:BIB_C3BC8F1EFDCA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy.
Journal
Optics letters
Author(s)
Shaffer E., Moratal C., Magistretti P., Marquet P., Depeursinge C.
ISSN
1539-4794 (Electronic)
ISSN-L
0146-9592
Publication state
Published
Issued date
15/12/2010
Peer-reviewed
Oui
Volume
35
Number
24
Pages
4102-4104
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy.
Keywords
Animals, Dermis/cytology, Epidermal Cells, Holography/methods, Image Processing, Computer-Assisted, Mice, Microscopy/methods, Molecular Imaging/methods
Pubmed
Web of science
Create date
11/03/2011 16:32
Last modification date
27/02/2024 8:17
Usage data