Hyperactivation of retina by light in mice leads to photoreceptor cell death mediated by VEGF and retinal pigment epithelium permeability.

Détails

Ressource 1Télécharger: BIB_C3B47D2E3C0C.P001.pdf (4537.20 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_C3B47D2E3C0C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Hyperactivation of retina by light in mice leads to photoreceptor cell death mediated by VEGF and retinal pigment epithelium permeability.
Périodique
Cell Death and Disease
Auteur(s)
Cachafeiro M., Bemelmans A.P., Samardzija M., Afanasieva T., Pournaras J.A., Grimm C., Kostic C., Philippe S., Wenzel A., Arsenijevic Y.
ISSN
2041-4889 (Electronic)
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
4
Pages
e781
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: epublish
Résumé
Light toxicity is suspected to enhance certain retinal degenerative processes such as age-related macular degeneration. Death of photoreceptors can be induced by their exposure to the visible light, and although cellular processes within photoreceptors have been characterized extensively, the role of the retinal pigment epithelium (RPE) in this model is less well understood. We demonstrate that exposition to intense light causes the immediate breakdown of the outer blood-retinal barrier (BRB). In a molecular level, we observed the slackening of adherens junctions tying up the RPE and massive leakage of albumin into the neural retina. Retinal pigment epithelial cells normally secrete vascular endothelial growth factor (VEGF) at their basolateral side; light damage in contrast leads to VEGF increase on the apical side - that is, in the neuroretina. Blocking VEGF, by means of lentiviral gene transfer to express an anti-VEGF antibody in RPE cells, inhibits outer BRB breakdown and retinal degeneration, as illustrated by functional, behavioral and morphometric analysis. Our data show that exposure to high levels of visible light induces hyperpermeability of the RPE, likely involving VEGF signaling. The resulting retinal edema contributes to irreversible damage to photoreceptors. These data suggest that anti-VEGF compounds are of therapeutic interest when the outer BRB is altered by retinal stresses.
Pubmed
Web of science
Open Access
Oui
Création de la notice
27/09/2013 10:33
Dernière modification de la notice
09/05/2019 0:54
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