Article: article from journal or magazin.
In vivo gene transfer into the ocular ciliary muscle mediated by ultrasound and microbubbles.
Ultrasound In Medicine and Biology
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
This study aimed to assess application of ultrasound (US) combined with microbubbles (MB) to transfect the ciliary muscle of rat eyes. Reporter DNA plasmids encoding for Gaussia luciferase, β-galactosidase or the green fluorescent protein (GFP), alone or mixed with 50% Artison MB, were injected into the ciliary muscle, with or without US exposure (US set at 1 MHz, 2 W/cm(2), 50% duty cycle for 2 min). Luciferase activity was measured in ocular fluids at 7 and 30 days after sonoporation. At 1 week, the US+MB treatment showed a significant increase in luminescence compared with control eyes, injected with plasmid only, with or without MB (×2.6), and, reporter proteins were localized in the ciliary muscle by histochemical analysis. At 1 month, a significant decrease in luciferase activity was observed in all groups. A rise in lens and ciliary muscle temperature was measured during the procedure but did not result in any observable or microscopic damages at 1 and 8 days. The feasibility to transfer gene into the ciliary muscle by US and MB suggests that sonoporation may allow intraocular production of proteins for the treatment of inflammatory, angiogenic and/or degenerative retinal diseases.
Analysis of Variance, Animals, Ciliary Body/cytology, Ciliary Body/metabolism, Eye Diseases/genetics, Eye Diseases/therapy, Feasibility Studies, Female, Gene Transfer Techniques, Green Fluorescent Proteins/genetics, Green Fluorescent Proteins/metabolism, In Situ Nick-End Labeling, Luciferases/genetics, Luciferases/metabolism, Microbubbles, Plasmids, Rats, Rats, Inbred Lew, Sonication, Statistics, Nonparametric, Transfection, beta-Galactosidase/genetics, beta-Galactosidase/metabolism
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