In vivo gene transfer into the ocular ciliary muscle mediated by ultrasound and microbubbles.

Détails

ID Serval
serval:BIB_C35325F78633
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
In vivo gene transfer into the ocular ciliary muscle mediated by ultrasound and microbubbles.
Périodique
Ultrasound In Medicine and Biology
Auteur(s)
Kowalczuk L., Boudinet M., El Sanharawi M., Touchard E., Naud M.C., Saïed A., Jeanny J.C., Behar-Cohen F., Laugier P.
ISSN
1879-291X (Electronic)
ISSN-L
0301-5629
Statut éditorial
Publié
Date de publication
2011
Peer-reviewed
Oui
Volume
37
Numéro
11
Pages
1814-1827
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
This study aimed to assess application of ultrasound (US) combined with microbubbles (MB) to transfect the ciliary muscle of rat eyes. Reporter DNA plasmids encoding for Gaussia luciferase, β-galactosidase or the green fluorescent protein (GFP), alone or mixed with 50% Artison MB, were injected into the ciliary muscle, with or without US exposure (US set at 1 MHz, 2 W/cm(2), 50% duty cycle for 2 min). Luciferase activity was measured in ocular fluids at 7 and 30 days after sonoporation. At 1 week, the US+MB treatment showed a significant increase in luminescence compared with control eyes, injected with plasmid only, with or without MB (×2.6), and, reporter proteins were localized in the ciliary muscle by histochemical analysis. At 1 month, a significant decrease in luciferase activity was observed in all groups. A rise in lens and ciliary muscle temperature was measured during the procedure but did not result in any observable or microscopic damages at 1 and 8 days. The feasibility to transfer gene into the ciliary muscle by US and MB suggests that sonoporation may allow intraocular production of proteins for the treatment of inflammatory, angiogenic and/or degenerative retinal diseases.
Mots-clé
Analysis of Variance, Animals, Ciliary Body/cytology, Ciliary Body/metabolism, Eye Diseases/genetics, Eye Diseases/therapy, Feasibility Studies, Female, Gene Transfer Techniques, Green Fluorescent Proteins/genetics, Green Fluorescent Proteins/metabolism, In Situ Nick-End Labeling, Luciferases/genetics, Luciferases/metabolism, Microbubbles, Plasmids, Rats, Rats, Inbred Lew, Sonication, Statistics, Nonparametric, Transfection, beta-Galactosidase/genetics, beta-Galactosidase/metabolism
Pubmed
Web of science
Création de la notice
19/08/2013 16:36
Dernière modification de la notice
19/03/2018 11:57
Données d'usage