Spatial organization of nucleotide excision repair proteins after UV-induced DNA damage in the human cell nucleus.

Details

Serval ID
serval:BIB_C33850CF0738
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Spatial organization of nucleotide excision repair proteins after UV-induced DNA damage in the human cell nucleus.
Journal
Journal of Cell Science
Author(s)
Solimando L., Luijsterburg M.S., Vecchio L., Vermeulen W., van Driel R., Fakan S.
ISSN
0021-9533
Publication state
Published
Issued date
2009
Volume
122
Number
Pt 1
Pages
83-91
Language
english
Notes
PDF type: Article
Abstract
Nucleotide excision repair (NER) is an evolutionary conserved DNA repair system that is essential for the removal of UV-induced DNA damage. In this study we investigated how NER is compartmentalized in the interphase nucleus of human cells at the ultrastructural level by using electron microscopy in combination with immunogold labeling. We analyzed the role of two nuclear compartments: condensed chromatin domains and the perichromatin region. The latter contains transcriptionally active and partly decondensed chromatin at the surface of condensed chromatin domains. We studied the distribution of the damage-recognition protein XPC and of XPA, which is a central component of the chromatin-associated NER complex. Both XPC and XPA rapidly accumulate in the perichromatin region after UV irradiation, whereas only XPC is also moderately enriched in condensed chromatin domains. These observations suggest that DNA damage is detected by XPC throughout condensed chromatin domains, whereas DNA-repair complexes seem preferentially assembled in the perichromatin region. We propose that UV-damaged DNA inside condensed chromatin domains is relocated to the perichromatin region, similar to what has been shown for DNA replication. In support of this, we provide evidence that UV-damaged chromatin domains undergo expansion, which might facilitate the translocation process. Our results offer novel insight into the dynamic spatial organization of DNA repair in the human cell nucleus.
Keywords
cell line , cell nucleus/metabolism , DNA/metabolism , DNA/radiation effects , DNA damage, DNA repair , DNA repair enzymes/metabolism , DNA-binding proteins/genetics , DNA-binding proteins/metabolism , humans , recombinant fusion proteins/genetics , recombinant fusion proteins/metabolism , xeroderma pigmentosum group A protein/genetics , xeroderma pigmentosum group A protein/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
12/10/2009 15:02
Last modification date
20/08/2019 15:38
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