Toxigenic C. difficile induced inflammatory marker expression by human intestinal epithelial cells is asymmetrical

Détails

ID Serval
serval:BIB_C32BC14F6BB5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Toxigenic C. difficile induced inflammatory marker expression by human intestinal epithelial cells is asymmetrical
Périodique
Life Sciences
Auteur(s)
Canny  G., Drudy  D., Macmathuna  P., O'Farrelly  C., Baird  A. W.
ISSN
0024-3205
Statut éditorial
Publié
Date de publication
01/2006
Peer-reviewed
Oui
Volume
78
Numéro
9
Pages
920-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 25
Résumé
Clostridium difficile infection of the intestinal epithelium and consequent pseudomembranous colitis is an important cause of morbidity and mortality. Pathogenesis has been ascribed exclusively to toxin production. Using in vitro models of human intestinal epithelial layers, we show that exposure to toxigenic C. difficile upregulates epithelial expression of IL-8 and ICAM-1, two molecules important in neutrophil chemoattraction and adhesion and subsequent inflammation. IL-8 production was also stimulated by toxin-containing supernatants. C difficile induced IL-8 release was inhibited by specific antiserum. Increased ICAM-1 expression only occurred after basolateral exposure to C. difficile while apical exposure had no effect on ICAM-1 expression. However, transepithelial electrical resistance was impaired by apical exposure to bacterial suspensions. We suggest that apical exposure to C. difficile induces changes in epithelial layer integrity which allows the bacteria and/or the toxin access to the basolateral compartment where pathogenic inflammatory mechanisms are activated.
Mots-clé
Antibodies, Blocking/pharmacology Bacterial Proteins/*toxicity Bacterial Toxins/*toxicity Biological Markers Clostridium difficile/*metabolism Electric Conductivity Enterotoxins/*toxicity Epithelial Cells/cytology/*metabolism HT29 Cells Humans Immunoassay Inflammation/*metabolism/microbiology Intercellular Adhesion Molecule-1/metabolism Interleukin-8/metabolism Intestinal Mucosa/cytology/*metabolism
Pubmed
Web of science
Création de la notice
18/01/2008 13:11
Dernière modification de la notice
03/03/2018 21:11
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