Monoclonal immunoglobulin A antibody directed against serotype-specific epitope of Shigella flexneri lipopolysaccharide protects against murine experimental shigellosis
Details
Serval ID
serval:BIB_C2B72399E153
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Monoclonal immunoglobulin A antibody directed against serotype-specific epitope of Shigella flexneri lipopolysaccharide protects against murine experimental shigellosis
Journal
Journal of Experimental Medicine
ISSN
0022-1007 (Print)
Publication state
Published
Issued date
09/1995
Volume
182
Number
3
Pages
769-78
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 1
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 1
Abstract
To determine the role of humoral mucosal immune response in protection against shigellosis, we have obtained a monoclonal dimeric immunoglobulin A (IgA) antibody specific for Shigella flexneri serotype 5a lipopolysaccharide (mIgA) and used a murine pulmonary infection model that mimics the lesions occurring in natural intestinal infection. Adult BALB/c mice challenged with 10(7) S. flexneri organisms developed a rapid inflammatory response characterized by polymorphonuclear cell infiltration around and within the bronchi and strong systemic interleukin 6 response. Implantation of hybridoma cells in the back of mice, resulting in the development of a myeloma tumor producing mIgA in the serum and subsequently secretory mIgA in local secretions, or direct intranasal administration of these antibodies, protected the animals against subsequent intranasal challenge with S. flexneri serotype 5a. Absence of histopathological lesion and significant decrease in bacterial load of the lungs and of systemic interleukin 6 response were the three major criteria of protection. This protection was shown to be serotype-specific and dependent on local concentration of mIgA. These data demonstrate that mucosal antibodies directed against a single polysaccharidic surface epitope of Shigella can protect against the disease.
Keywords
Administration, Intranasal
Animals
Antibodies, Bacterial/administration & dosage/*immunology
Antibodies, Monoclonal/administration & dosage/*immunology
Disease Models, Animal
Dysentery, Bacillary/blood/microbiology/pathology/*prevention & control
Epitopes/*immunology
Female
Hybridomas/immunology/transplantation
*Immunization, Passive
Immunoglobulin A/administration & dosage/*immunology
Interleukin-6/blood
Lipopolysaccharides/*immunology
Lung/microbiology/pathology
Mice
Mice, Inbred BALB C
Pneumonia, Bacterial/blood/microbiology/pathology/*prevention & control
Serotyping
Shigella flexneri/classification/*immunology
Species Specificity
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 17:01
Last modification date
20/08/2019 16:37