DNA methylation by CcrM activates the transcription of two genes required for the division of Caulobacter crescentus.

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_C28ECAE2773B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
DNA methylation by CcrM activates the transcription of two genes required for the division of Caulobacter crescentus.
Périodique
Molecular Microbiology
Auteur(s)
Gonzalez D., Collier J.
ISSN
1365-2958 (Electronic)
ISSN-L
0950-382X
Statut éditorial
Publié
Date de publication
2013
Volume
88
Numéro
1
Pages
203-218
Langue
anglais
Résumé
DNA methylation regulates many processes, including gene expression, by superimposing secondary information on DNA sequences. The conserved CcrM enzyme, which methylates adenines in GANTC sequences, is essential to the viability of several Alphaproteobacteria. In this study, we find that Caulobacter crescentus cells lacking the CcrM enzyme accumulate low levels of the two conserved FtsZ and MipZ proteins, leading to a severe defect in cell division. This defect can be compensated by the expression of the ftsZ gene from an inducible promoter or by spontaneous suppressor mutations that promote FtsZ accumulation. We show that CcrM promotes the transcription of the ftsZ and mipZ genes and that the ftsZ and mipZ promoter regions contain a conserved CGACTC motif that is critical to their activities and to their regulation by CcrM. In addition, our results suggest that the ftsZ promoter has the lowest activity when the CGACTC motif is non-methylated, an intermediate activity when it is hemi-methylated and the highest activity when it is fully methylated. The regulation of ftsZ expression by DNA methylation may explain why CcrM is essential in a subset of Alphaproteobacteria.
Pubmed
Web of science
Open Access
Oui
Création de la notice
14/03/2013 14:27
Dernière modification de la notice
20/08/2019 16:37
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