Interplay of DNA supercoiling and catenation during the segregation of sister duplexes.

Détails

Ressource 1Télécharger: BIB_C26EB08DD54C.P001.pdf (6752.94 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_C26EB08DD54C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Interplay of DNA supercoiling and catenation during the segregation of sister duplexes.
Périodique
Nucleic Acids Research
Auteur(s)
Martínez-Robles M.L., Witz G., Hernández P., Schvartzman J.B., Stasiak A., Krimer D.B.
ISSN
1362-4962[electronic], 0305-1048[linking]
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
37
Numéro
15
Pages
5126-5137
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The discrete regulation of supercoiling, catenation and knotting by DNA topoisomerases is well documented both in vivo and in vitro, but the interplay between them is still poorly understood. Here we studied DNA catenanes of bacterial plasmids arising as a result of DNA replication in Escherichia coli cells whose topoisomerase IV activity was inhibited. We combined high-resolution two-dimensional agarose gel electrophoresis with numerical simulations in order to better understand the relationship between the negative supercoiling of DNA generated by DNA gyrase and the DNA interlinking resulting from replication of circular DNA molecules. We showed that in those replication intermediates formed in vivo, catenation and negative supercoiling compete with each other. In interlinked molecules with high catenation numbers negative supercoiling is greatly limited. However, when interlinking decreases, as required for the segregation of newly replicated sister duplexes, their negative supercoiling increases. This observation indicates that negative supercoiling plays an active role during progressive decatenation of newly replicated DNA molecules in vivo.
Mots-clé
DNA Gyrase/metabolism, DNA Replication, DNA, Catenated/analysis, DNA, Catenated/chemistry, DNA, Superhelical/analysis, DNA, Superhelical/chemistry, Electrophoresis, Gel, Two-Dimensional, Escherichia coli/genetics, Models, Molecular
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/11/2009 13:28
Dernière modification de la notice
09/05/2019 0:49
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