A conserved transcript pattern in response to a specialist and a generalist herbivore.

Details

Serval ID
serval:BIB_C2606A6218A3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A conserved transcript pattern in response to a specialist and a generalist herbivore.
Journal
Plant Cell
Author(s)
Reymond P., Bodenhausen N., Van Poecke R.M., Krishnamurthy V., Dicke M., Farmer E.E.
ISSN
1040-4651 (Print)
ISSN-L
1040-4651
Publication state
Published
Issued date
2004
Volume
16
Number
11
Pages
3132-3147
Language
english
Abstract
Transcript patterns elicited in response to attack reveal, at the molecular level, how plants respond to aggressors. These patterns are fashioned both by inflicted physical damage as well as by biological components displayed or released by the attacker. Different types of attacking organisms might therefore be expected to elicit different transcription programs in the host. Using a large-scale DNA microarray, we characterized gene expression in damaged as well as in distal Arabidopsis thaliana leaves in response to the specialist insect, Pieris rapae. More than 100 insect-responsive genes potentially involved in defense were identified, including genes involved in pathogenesis, indole glucosinolate metabolism, detoxification and cell survival, and signal transduction. Of these 114 genes, 111 were induced in Pieris feeding, and only three were repressed. Expression patterns in distal leaves were markedly similar to those of local leaves. Analysis of wild-type and jasmonate mutant plants, coupled with jasmonate treatment, showed that between 67 and 84% of Pieris-regulated gene expression was controlled, totally or in part, by the jasmonate pathway. This was correlated with increased larval performance on the coronatine insensitive1 glabrous1 (coi1-1 gl1) mutant. Independent mutations in COI1 and GL1 led to a faster larval weight gain, but the gl1 mutation had relatively little effect on the expression of the insect-responsive genes examined. Finally, we compared transcript patterns in Arabidopis in response to larvae of the specialist P. rapae and to a generalist insect, Spodoptera littoralis. Surprisingly, given the complex nature of insect salivary components and reported differences between species, almost identical transcript profiles were observed. This study also provides a robustly characterized gene set for the further investigation of plant-insect interaction.
Keywords
Animals, Arabidopsis/genetics, Arabidopsis/physiology, Butterflies, Conserved Sequence, Cyclopentanes/metabolism, Food Chain, Gene Expression Regulation, Plant/physiology, Larva, Multigene Family/physiology, Oligonucleotide Array Sequence Analysis, Oxylipins, Spodoptera, Transcription, Genetic/physiology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 21:05
Last modification date
20/08/2019 16:37
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