Detection of Reactive Oxygen Species Using MitoSOX and CellROX in Zebrafish

Détails

ID Serval
serval:BIB_C1F022A936E1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Detection of Reactive Oxygen Species Using MitoSOX and CellROX in Zebrafish
Périodique
Bio-Protocol
Auteur(s)
Rissone Alberto, Candotti Fabio
ISSN
2331-8325
Statut éditorial
Publié
Date de publication
05/10/2016
Peer-reviewed
Oui
Volume
6
Numéro
19
Pages
NA
Langue
anglais
Résumé
The production of free radicals is the result of normal cellular metabolism. Free radicals are involved in innumerable different cellular and biological functions such as signaling, proliferation, cell death, aging, inflammation, etc. Under physiological conditions, the levels of reactive oxygen species (ROS) are strictly regulated by the cells. However, during stressful conditions such as oxidative stress, ROS levels increase causing damages to different molecules like DNA, lipids and proteins. Increased levels of ROS have been associated with a growing list of different diseases. In this protocol, we used MitoSOX and CellROX Green oxidative stress probes to label the intracellular ROS and detect the fluorescence using cell sorting and confocal analyses.
Création de la notice
23/10/2017 12:27
Dernière modification de la notice
03/03/2018 21:08
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