Cadmium uptake by brush border membrane vesicles from the rabbit renal external cortex

Détails

ID Serval
serval:BIB_C1CE61F12089
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cadmium uptake by brush border membrane vesicles from the rabbit renal external cortex
Périodique
Journal of Trace Elements and Electrolytes in Health and Disease
Auteur(s)
Mingard  F., Diezi  J.
ISSN
0931-2838
Statut éditorial
Publié
Date de publication
06/1992
Volume
6
Numéro
2
Pages
111-5
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
Brush border membrane vesicles (BBMV) isolated from the rabbit renal external cortex were used to investigate inorganic cadmium (Cd) uptake. BBMV were incubated during 1 to 60 min in a buffered solution containing 10 to 200 mumol/L Cd (CdCl2), and uptake was determined using a rapid filtration technique. Cd uptake by BBMV was time-and concentration-dependent. At the lowest concentration (10 mumol/L), Cd uptake was nearly complete (70% of the plateau value, which was obtained after 10 min) within 1 min. At the highest concentration (200 mumol/L), the percentage of Cd uptake during the first minute was smaller (50%) and a slower component of uptake appeared. From additional experiments where Cd-loaded BBMV were submitted to an osmotic shock or to variations of extravesicular osmolality, it was concluded that the uptake of Cd by BBMV resulted essentially from membrane binding and not from intravesicular accumulation. The change in pH from 7.4 to 5.8 under iso- or hypotonic conditions removed about 40% of Cd bound to the BBMV, indicating that Cd binding to the membrane is pH sensitive. Thus, binding to the brush border membrane constitutes an initial step for inorganic Cd entry into renal epithelial cells.
Mots-clé
Animals Cadmium/*pharmacokinetics Cell Membrane/metabolism Female Hydrogen-Ion Concentration Kidney Cortex/*metabolism Male Microvilli/metabolism Rabbits
Pubmed
Web of science
Création de la notice
21/01/2008 12:54
Dernière modification de la notice
03/03/2018 21:08
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