A thin chip microsprayer system coupled to Fourier transform ion cyclotron resonance mass spectrometry for glycopeptide screening.

Details

Serval ID
serval:BIB_C1B6E9005BC3
Type
Article: article from journal or magazin.
Collection
Publications
Title
A thin chip microsprayer system coupled to Fourier transform ion cyclotron resonance mass spectrometry for glycopeptide screening.
Journal
Rapid Communications In Mass Spectrometry : Rcm
Author(s)
Bindila L., Froesch M., Lion N., Vukelić Z., Rossier J.S., Girault H.H., Peter-Katalinić J., Zamfir A.D.
ISSN
0951-4198 (Print)
ISSN-L
0951-4198
Publication state
Published
Issued date
2004
Peer-reviewed
Oui
Volume
18
Number
23
Pages
2913-2920
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
A thin polymer microchip was coupled with a Fourier transform ion cyclotron resonance (FTICR) 9.4 T mass spectrometer and the method was optimized in negative ion mode for glycopeptide screening. The interface between the polymer microchip and FTICR mass spectrometer consists of an in-laboratory conceived and designed mounting system that exhibits robust and controllable alignment of the chip toward the inlet of the mass spectrometer. The particular attribute of the polymer chip coupled to the FTICR mass spectrometer, to achieve an increase in ionization efficiency and sensitivity under the premise of high mass accuracy of detection, is highlighted by the large number of major and minor glycopeptide structures detected and identified in highly heterogeneous mixtures obtained from urine matrices. Glycoforms expressing various saccharide chain lengths ranging from tri- to dodecasaccharide, bearing up to three sialic acid moieties, could be detected and assigned based on the accuracy of the mass measurement (average mass deviation below 6 ppm) of their molecular ions. -Thin chipESI-FTICRMS is a potent novel system for glycomic screening of complex mixtures, as demonstrated for identification of singly sialylated O-glycosylated amino acids and peptides from urine matrices, and could be considered for general applicability in the glycoanalytical field.
Keywords
Amino Acid Sequence, Carbohydrate Sequence, Child, Cyclotrons, Glycopeptides/analysis, Glycopeptides/classification, Humans, Lysosomal Storage Diseases/diagnosis, Lysosomal Storage Diseases/urine, Microchemistry/methods, Miniaturization, Molecular Sequence Data, Reproducibility of Results, Sensitivity and Specificity, Sequence Analysis, Protein/instrumentation, Sequence Analysis, Protein/methods, Spectroscopy, Fourier Transform Infrared/instrumentation, Spectroscopy, Fourier Transform Infrared/methods
Pubmed
Web of science
Create date
09/11/2014 23:49
Last modification date
20/08/2019 16:36
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