Fading and rebound of P2X2 currents at millimolar ATP concentrations caused by low pH.

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_C1A78ED8BEF1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Fading and rebound of P2X2 currents at millimolar ATP concentrations caused by low pH.
Périodique
British Journal of Pharmacology
Auteur(s)
Stoop R., Quayle J.M.
ISSN
0007-1188 (Print)
ISSN-L
0007-1188
Statut éditorial
Publié
Date de publication
1998
Peer-reviewed
Oui
Volume
125
Numéro
2
Pages
235-237
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
When ATP is applied at high concentrations (above 1 mM) to PC12 cells, it produces a rapidly desensitizing peak current followed by a rebound of the current after termination of the ATP application. We expressed P2X2 receptors, which are thought to mediate the ATP currents of PC12 cells, in HEK293 cells and studied the effects of acidification on this 'fading and rebound' phenomenon. We found that the desensitization disappeared after adjusting the low pH (<5.0) of the millimolar ATP concentrations to a more physiological value (pH 7.3). Furthermore, the fading and rebound could also be induced at much lower ATP concentrations by decreasing the pH of the ATP containing application solutions. Thus, it appears this phenomenon is not caused directly by high concentrations of ATP, but is due to a concomitant acidification that occurs when high concentrations of ATP are dissolved in only moderately buffered application solutions.
Mots-clé
Adenosine Triphosphate/metabolism, Animals, Hydrogen-Ion Concentration, Ligands, PC12 Cells, Rats, Receptors, Purinergic P2/drug effects, Receptors, Purinergic P2/metabolism, Receptors, Purinergic P2X2, Tumor Cells, Cultured
Pubmed
Open Access
Oui
Création de la notice
29/10/2013 10:42
Dernière modification de la notice
20/08/2019 15:36
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