Collecting duct-specific gene inactivation of alphaENaC in the mouse kidney does not impair sodium and potassium balance

Détails

ID Serval
serval:BIB_C1951B21B5F6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Collecting duct-specific gene inactivation of alphaENaC in the mouse kidney does not impair sodium and potassium balance
Périodique
Journal of Clinical Investigation
Auteur(s)
Rubera  I., Loffing  J., Palmer  L. G., Frindt  G., Fowler-Jaeger  N., Sauter  D., Carroll  T., McMahon  A., Hummler  E., Rossier  B. C.
ISSN
0021-9738
Statut éditorial
Publié
Date de publication
08/2003
Peer-reviewed
Oui
Volume
112
Numéro
4
Pages
554-65
Notes
Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. --- Old month value: Aug
Résumé
Aldosterone controls the final sodium reabsorption and potassium secretion in the kidney by regulating the activity of the epithelial sodium channel (ENaC) in the aldosterone-sensitive distal nephron (ASDN). ASDN consists of the last portion of the distal convoluted tubule (late DCT), the connecting tubule (CNT), and the collecting duct (CD) (i.e., the cortical CD [CCD] and the medullary CD [MCD]). It has been proposed that the control of sodium transport in the CCD is essential for achieving sodium and potassium balance. We have tested this hypothesis by inactivating the alpha subunit of ENaC in the CD but leaving ENaC expression in the late DCT and CNT intact. Under salt restriction or under aldosterone infusion, whole-cell voltage clamp of principal cells of CCD showed no detectable ENaC activity, whereas large amiloride-sensitive currents were observed in control littermates. The animals survive well and are able to maintain sodium and potassium balance, even when challenged by salt restriction, water deprivation, or potassium loading. We conclude that the expression of ENaC in the CD is not a prerequisite for achieving sodium and potassium balance in mice. This stresses the importance of more proximal nephron segments (late DCT/CNT) to achieve sodium and potassium balance.
Mots-clé
Aldosterone/blood Animals Electrophysiology Epithelial Sodium Channel Homeodomain Proteins/metabolism Immunohistochemistry Kidney Tubules/metabolism/*physiology Kidney Tubules, Collecting/metabolism/*physiology Mice Mice, Knockout Microscopy, Fluorescence Potassium/metabolism Promoter Regions (Genetics) Protein Structure, Tertiary RNA, Messenger/metabolism Salts/metabolism Sodium/metabolism Sodium Channels/*genetics/*physiology Time Factors Water/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 14:00
Dernière modification de la notice
09/05/2019 0:46
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