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Detection of serum antibodies against Leishmania 94 kDa antigen in visceral and cutaneous leishmaniosis due to Leishmania infantum.
Leishmania promastigotes polypeptides are analyzed by immunoblotting with sera from patients infected with different Leishmania species and presenting visceral or cutaneous infections. These sera recognize Leishmania polypeptides in several molecular masses. The major findings of this study are as follow. 1) The Leishmania 94 kDa antigen, which is specifically recognized by all sera from L. infantum-infected patients with visceral infection, is recognized by some sera from L. infantum-infected patients presenting cutaneous infection. 2) All patients with cutaneous infections due to L. tropica, L. amazonensis, or L. guyanensis do not develop anti-94 kDa antibodies, whatever the Leishmania species used as antigens. 3) Difference in electrophoretic mobilities is seen between the 94 kDa antigen identified by sera from Leishmania infantum-infected patients, and the antigen both recognized by the Concavalin A lectin and a rabbit antiserum raised against deglycosylated Promastigote Surface Protease.
Adult, Aged, Aged, 80 and over, Animals, Antibodies, Protozoan/blood, Antigens, Protozoan/chemistry, Antigens, Protozoan/immunology, Blotting, Western, Electrophoresis, Polyacrylamide Gel, Fluorescent Antibody Technique, Indirect, Humans, Immunoglobulins/blood, Leishmania/immunology, Leishmania infantum/immunology, Leishmaniasis, Cutaneous/immunology, Leishmaniasis, Cutaneous/parasitology, Leishmaniasis, Visceral/immunology, Leishmaniasis, Visceral/parasitology, Male, Middle Aged, Molecular Weight
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