Six and Eya expression during human somitogenesis and MyoD gene family activation

Détails

ID Serval
serval:BIB_C16611169B69
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Six and Eya expression during human somitogenesis and MyoD gene family activation
Périodique
Journal of Muscle Research and Cell Motility
Auteur(s)
Fougerousse  F., Durand  M., Lopez  S., Suel  L., Demignon  J., Thornton  C., Ozaki  H., Kawakami  K., Barbet  P., Beckmann  J. S., Maire  P.
ISSN
0142-4319 (Print)
Statut éditorial
Publié
Date de publication
2002
Volume
23
Numéro
3
Pages
255-64
Notes
Journal Article
Research Support, Non-U.S. Gov't
Résumé
This report describes the characterisation of the expression profile of several myogenic determination genes during human embryogenesis. The data were obtained from axial structures and limb buds of human embryos aged between 3 and 8 weeks of development. Using in situ hybridisation to detect Pax3 and MyoD gene family mRNAs, and immunochemistry to follow Six and Eya protein accumulation, we have been able to establish the chronology of accumulation of these gene products. As in mouse, the first transcripts detected in myotomes of 3 week-old embryos are Pax3 and Myf5, followed by the expression of myogenin. MyoD appears to be activated well after Myf5, myogenin and MRF4 in the early myotome, whereas, in limb bud muscles, the presence of all four of these mRNAs is concomitant from 6 weeks. Six1, Six4 and Six5 homeoproteins are detected later than Myf5 activation. These Six homeoproteins are first observed in the cytoplasm of myogenin expressing cells. At later stages of development, Six1 and Six5, but not Six4, are translocated into the nuclei of myogenic cells, concomitantly with MyHCemb expression. Eya1 and Eya2 proteins, potential Six cofactors, were also detected in myogenin positive cells, but their accumulation was delayed and was mainly cytoplasmic. These results preclude that early activation of Myf5, myogenin and MRF4 is under the control of Six and Eya proteins, while Six and Eya proteins would be involved in later steps of myogenic differentiation.
Mots-clé
Body Patterning/genetics Cell Differentiation/*genetics Cell Nucleus/genetics/metabolism Cytoplasm/genetics/metabolism DNA-Binding Proteins/genetics Gene Expression Regulation, Developmental/*genetics Homeodomain Proteins/*metabolism Humans Immunohistochemistry Intracellular Signaling Peptides and Proteins Muscle Proteins/genetics Muscle, Skeletal/cytology/*embryology/metabolism MyoD Protein/*genetics Myogenic Regulatory Factor 5 Myogenic Regulatory Factors/genetics Myogenin/genetics Nuclear Proteins Paired Box Transcription Factors Protein Isoforms/genetics/metabolism Protein Transport/genetics Protein-Tyrosine-Phosphatase RNA, Messenger/metabolism Somites/cytology/*metabolism Trans-Activators/*metabolism *Transcription Factors
Pubmed
Web of science
Création de la notice
25/01/2008 17:18
Dernière modification de la notice
03/03/2018 21:07
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