Cloning of murine interferon gamma receptor cDNA: expression in human cells mediates high-affinity binding but is not sufficient to confer sensitivity to murine interferon gamma.

Détails

ID Serval
serval:BIB_C143EC26A88A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cloning of murine interferon gamma receptor cDNA: expression in human cells mediates high-affinity binding but is not sufficient to confer sensitivity to murine interferon gamma.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Hemmi S., Peghini P., Metzler M., Merlin G., Dembic Z., Aguet M.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
1989
Volume
86
Numéro
24
Pages
9901-9905
Langue
anglais
Résumé
A full-length cDNA encoding the murine interferon gamma (IFN-gamma) receptor was isolated from a lambda gt11 library using a human IFN-gamma receptor cDNA probe. The deduced amino acid sequence of the murine IFN-gamma receptor shows approximately 53% homology to its human counterpart but no homology to other known proteins. Murine IFN-gamma receptor cDNA was expressed in human HEp-2 cells, which do not bind murine IFN-gamma and are insensitive to its action. Transfectants displayed the same binding properties as mouse cells. The biological responsiveness of such transfectants to various biological effects of both human and murine IFN-gamma was investigated, including modulation of major histocompatibility complex class I and class II antigen expression, inhibition of cell growth, and antiviral activity. Like parental HEp-2 cells, these transfectants responded only to human, but not to murine, IFN-gamma. Inversely, mouse L929 cells transfected with human IFN-gamma receptor cDNA were insensitive to human IFN-gamma. These results confirm and extend previous findings, suggesting that species-specific cofactors are needed for IFN-gamma-mediated signal transduction.
Mots-clé
Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cloning, Molecular, DNA/genetics, DNA/isolation &amp, purification, Gene Expression, Genes, Humans, Interferon-gamma/metabolism, Kinetics, L Cells (Cell Line)/immunology, Major Histocompatibility Complex, Mice, Molecular Sequence Data, Plasmids, Receptors, Immunologic/genetics, Receptors, Immunologic/metabolism, Receptors, Interferon, Recombinant Proteins/metabolism, Sequence Homology, Nucleic Acid, Transfection
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:37
Dernière modification de la notice
09/05/2019 0:45
Données d'usage