Systematic analysis of complex genetic interactions.

Détails

ID Serval
serval:BIB_C1022D51E7B4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Systematic analysis of complex genetic interactions.
Périodique
Science
Auteur(s)
Kuzmin E., VanderSluis B., Wang W., Tan G., Deshpande R., Chen Y., Usaj M., Balint A., Mattiazzi Usaj M., van Leeuwen J., Koch E.N., Pons C., Dagilis A.J., Pryszlak M., Wang JZY, Hanchard J., Riggi M., Xu K., Heydari H., San Luis B.J., Shuteriqi E., Zhu H., Van Dyk N., Sharifpoor S., Costanzo M., Loewith R., Caudy A., Bolnick D., Brown G.W., Andrews B.J., Boone C., Myers C.L.
ISSN
1095-9203 (Electronic)
ISSN-L
0036-8075
Statut éditorial
Publié
Date de publication
20/04/2018
Peer-reviewed
Oui
Volume
360
Numéro
6386
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Résumé
To systematically explore complex genetic interactions, we constructed ~200,000 yeast triple mutants and scored negative trigenic interactions. We selected double-mutant query genes across a broad spectrum of biological processes, spanning a range of quantitative features of the global digenic interaction network and tested for a genetic interaction with a third mutation. Trigenic interactions often occurred among functionally related genes, and essential genes were hubs on the trigenic network. Despite their functional enrichment, trigenic interactions tended to link genes in distant bioprocesses and displayed a weaker magnitude than digenic interactions. We estimate that the global trigenic interaction network is ~100 times as large as the global digenic network, highlighting the potential for complex genetic interactions to affect the biology of inheritance, including the genotype-to-phenotype relationship.
Mots-clé
Gene Regulatory Networks, Mutation, Oligonucleotide Array Sequence Analysis, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins/genetics
Pubmed
Web of science
Création de la notice
22/01/2019 17:08
Dernière modification de la notice
20/08/2019 16:35
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