A highly sensitive LC-tandem MS assay for the measurement in plasma and in urine of salbutamol administered by nebulization during mechanical ventilation in healthy volunteers.

Détails

ID Serval
serval:BIB_C0AEA0A28D92
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A highly sensitive LC-tandem MS assay for the measurement in plasma and in urine of salbutamol administered by nebulization during mechanical ventilation in healthy volunteers.
Périodique
Biomedical Chromatography : BMC
Auteur(s)
Sidler-Moix A.L., Mercier T., Decosterd L.A., Di Paolo E.R., Berger-Gryllaki M., Cotting J., Pannatier A.
ISSN
1099-0801 (Electronic)
ISSN-L
0269-3879
Statut éditorial
Publié
Date de publication
2012
Peer-reviewed
Oui
Volume
26
Numéro
5
Pages
672-680
Langue
anglais
Notes
Publication types: Journal Article
Résumé
The new-generation nebulizers are commonly used for the administration of salbutamol in mechanically ventilated patients. The different modes of administration and new devices have not been compared. We developed a liquid chromatography-tandem mass spectrometry method for the determination of concentrations as low as 0.05 ng/mL of salbutamol, corresponding to the desired plasma concentration after inhalation. Salbutamol quantification was performed by reverse-phase HPLC. Analyte quantification was performed by electrospray ionization-triple quadrupole mass spectrometry using selected reaction monitoring detection ESI in the positive mode. The method was validated over concentrations ranging from 0.05 to 100 ng/mL in plasma and from 0.18 to 135 ng/mL in urine. The method is precise, with mean inter-day coefficient of variation (CV%) within 3.1-8.3% in plasma and 1.3-3.9% in urine, as well as accurate. The proposed method was found to reach the required sensitivity for the evaluation of different nebulizers as well as nebulization modes. The present assay was applied to examine whether salbutamol urine levels, normalized with the creatinine levels, correlated with the plasma concentrations. A suitable, convenient and noninvasive method of monitoring patients receiving salbutamol by mechanical ventilation could be implemented. Copyright © 2011 John Wiley & Sons, Ltd.
Pubmed
Web of science
Création de la notice
28/04/2012 9:25
Dernière modification de la notice
03/03/2018 21:06
Données d'usage