Article: article from journal or magazin.
Stimuliatsiia vazopressinom gidroliza fasfatidilinozit-4,5-difosfata i ego sviaz' s biosintezom prostaglandinov v épitelii mochevogo puzyria liagushki [Stimulation by vasopressin of hydrolysis of phosphatidylinositol- 4,5-diphosphate and its relation to prostaglandin biosynthesis in the bladder epithelium of frogs].
Biulleten' Eksperimental'noĭ Biologii i Meditsiny
In experiments carried out on the frog urinary bladder, it was found that 20 sec after vasopressin was added, the content of 1,2-di-acylglycerol, labelled with [3H]-arachidonic acid, increased by 44% and the content of [3H]-phosphatidylinositol-4,5-diphosphate (PIP2) decreased by 22%. Five minutes after hormone addition the amount of prostaglandin E (PGE) released into the serosal solution was increased three-fold. Preincubation of bladders in 10(-4) M neomycin led to a 26% increase in vasopressin-stimulated water flow, a block of PIP2 breakdown, and a reduction in PGE synthesis of 62%. A significant decrease in content of lipids labelled with [3H]-arachidonic acid was found in 1,2-diacylglycerol and phosphatidylethanolamine (diacyl form). The data obtained suggest that the role of PIP2 breakdown products in negative feed-back regulation of the hydroosmotic action of vasopressin at least in part includes their connection with PGE biosynthesis activation.
Animals, Epithelium/metabolism, Hydrolysis/drug effects, Phosphatidylinositols/metabolism, Prostaglandins E/biosynthesis, Rana temporaria, Stimulation, Chemical, Urinary Bladder/drug effects, Urinary Bladder/metabolism, Vasopressins/pharmacology
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