The Rab3-interacting molecule RIM is expressed in pancreatic beta-cells and is implicated in insulin exocytosis

Détails

ID Serval
serval:BIB_C08FD241B2E6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The Rab3-interacting molecule RIM is expressed in pancreatic beta-cells and is implicated in insulin exocytosis
Périodique
FEBS Letters
Auteur(s)
Iezzi  M., Regazzi  R., Wollheim  C. B.
ISSN
0014-5793 (Print)
Statut éditorial
Publié
Date de publication
05/2000
Volume
474
Numéro
1
Pages
66-70
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May 26
Résumé
The putative Rab3 effector RIM (Rab3-interacting molecule) was detected by Northern blotting, RT-PCR and Western blotting in native pancreatic beta-cells as well as in the derived cell lines INS-1E and HIT-T15. RIM was localized on the plasma membrane of INS-1E cells and beta-cells. An involvement of RIM in insulin exocytosis was indicated by transfection experiments of INS-1E cells with the Rab3 binding domain of RIM. This domain enhanced glucose-stimulated secretion in intact cells and Ca(2+)-stimulated exocytosis in permeabilized cells. Co-expression of Rab3A reversed the effect of RIM on exocytosis. These results suggest an implication of RIM in the control of insulin secretion.
Mots-clé
Animals Cell Line Cell Membrane/chemistry Cricetinae *Exocytosis *GTP-Binding Proteins *Gene Expression Humans Insulin/*secretion Insulinoma Islets of Langerhans/*metabolism/ultrastructure Mice Nerve Tissue Proteins/analysis/*genetics/*physiology Pancreatic Neoplasms Rats Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured rab3 GTP-Binding Proteins/metabolism
Pubmed
Web of science
Création de la notice
24/01/2008 15:30
Dernière modification de la notice
03/03/2018 21:06
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