Secretion of bioactive granulocyte-macrophage colony-stimulating factor by human colorectal carcinoma cells

Détails

ID Serval
serval:BIB_C078E4901F1B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Secretion of bioactive granulocyte-macrophage colony-stimulating factor by human colorectal carcinoma cells
Périodique
Cancer Research
Auteur(s)
Lahm  H., Wyniger  J., Hertig  S., Yilmaz  A., Fischer  J. R., Givel  J. C., Odartchenko  N.
ISSN
0008-5472 (Print)
Statut éditorial
Publié
Date de publication
07/1994
Volume
54
Numéro
14
Pages
3700-2
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul 15
Résumé
Secretion of several cytokines by colorectal carcinoma cells has been substantiated. These do not include granulocyte-macrophage colony-stimulating factor (GM-CSF) thus far. We show that the supernatant of two human colorectal carcinoma cell lines, LS1034 and SW480, stimulates proliferation of GM-CSF-dependent M07e cells. The activity was constitutively secreted by LS1034 cells and could be induced by serum-free culture conditions in SW480 cells. Addition of a neutralizing anti-GM-CSF antibody completely inhibited this activity. Preabsorption with anti-GM-CSF antibody removed all M07e growth-stimulating activity from LS1034 and SW480 supernatant. Western blot analysis revealed the presence of GM-CSF in LS1034 supernatant. Our results indicate that human colorectal carcinoma cells secrete indeed biologically active GM-CSF.
Mots-clé
Colorectal Neoplasms/pathology/*secretion Granulocyte-Macrophage Colony-Stimulating Factor/*secretion Humans Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
28/01/2008 9:56
Dernière modification de la notice
03/03/2018 21:05
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